Application of copper ion transporter gene OsCOPT7 in rice breeding
The invention discloses application of a copper ion transporter gene OsCOPT7 in rice breeding, and belongs to the field of plant genetic engineering. The amino acid sequence of the protein encoded by the OsCOPT7 gene is as shown in SEQ ID NO. 1, and the CDS sequence is as shown in SEQ ID NO. 2. By c...
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| creator | LI QIN YUAN FEIRONG LUO MENG LYU KAI LIAN XINGMING |
| description | The invention discloses application of a copper ion transporter gene OsCOPT7 in rice breeding, and belongs to the field of plant genetic engineering. The amino acid sequence of the protein encoded by the OsCOPT7 gene is as shown in SEQ ID NO. 1, and the CDS sequence is as shown in SEQ ID NO. 2. By constructing OsCOPT7 gene overexpression (OE) and knockout (CRISPR) transgenic materials, it is found that the yield of a single plant of an OsCOPT7 overexpression family is decreased, the yield of an OsCOPT7 knockout family is remarkably increased, and the rice yield is increased by conducting deletion mutation on a coding region of the gene. Therefore, the OsCOPT7 gene can be used in rice breeding to improve the rice yield, and has a good application prospect in genetic engineering breeding.
本发明公开了铜离子转运蛋白基因OsCOPT7在水稻选育中的应用，属于植物基因工程领域。OsCOPT7基因编码蛋白质的氨基酸序列如SEQ ID NO.1所示，CDS序列如SEQ ID NO.2所示。本发明通过构建OsCOPT7基因超表达(OE)和敲除(CRISPR)转基因材料，发现OsCOPT7超表达家系单株产量下降，而OsCOPT7敲除家系产量显著提高，通过将该基因的编码区进行缺失突变提高了水稻产量。因此，OsCOPT7基因可用于水稻选育中以提高水 |
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本发明公开了铜离子转运蛋白基因OsCOPT7在水稻选育中的应用，属于植物基因工程领域。OsCOPT7基因编码蛋白质的氨基酸序列如SEQ ID NO.1所示，CDS序列如SEQ ID NO.2所示。本发明通过构建OsCOPT7基因超表达(OE)和敲除(CRISPR)转基因材料，发现OsCOPT7超表达家系单株产量下降，而OsCOPT7敲除家系产量显著提高，通过将该基因的编码区进行缺失突变提高了水稻产量。因此，OsCOPT7基因可用于水稻选育中以提高水</description><language>chi ; eng</language><subject>AGRICULTURE ; ANIMAL HUSBANDRY ; BEER ; BIOCHEMISTRY ; CHEMISTRY ; COMPOSITIONS THEREOF ; CULTURE MEDIA ; ENZYMOLOGY ; FISHING ; FORESTRY ; HUMAN NECESSITIES ; HUNTING ; METALLURGY ; MICROBIOLOGY ; MICROORGANISMS OR ENZYMES ; MUTATION OR GENETIC ENGINEERING ; NEW PLANTS OR PROCESSES FOR OBTAINING THEM ; ORGANIC CHEMISTRY ; PEPTIDES ; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES ; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS ; SPIRITS ; TRAPPING ; VINEGAR ; WINE</subject><creationdate>2022</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20220318&DB=EPODOC&CC=CN&NR=114196679A$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,780,885,25564,76547</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20220318&DB=EPODOC&CC=CN&NR=114196679A$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>LI QIN</creatorcontrib><creatorcontrib>YUAN FEIRONG</creatorcontrib><creatorcontrib>LUO MENG</creatorcontrib><creatorcontrib>LYU KAI</creatorcontrib><creatorcontrib>LIAN XINGMING</creatorcontrib><title>Application of copper ion transporter gene OsCOPT7 in rice breeding</title><description>The invention discloses application of a copper ion transporter gene OsCOPT7 in rice breeding, and belongs to the field of plant genetic engineering. The amino acid sequence of the protein encoded by the OsCOPT7 gene is as shown in SEQ ID NO. 1, and the CDS sequence is as shown in SEQ ID NO. 2. By constructing OsCOPT7 gene overexpression (OE) and knockout (CRISPR) transgenic materials, it is found that the yield of a single plant of an OsCOPT7 overexpression family is decreased, the yield of an OsCOPT7 knockout family is remarkably increased, and the rice yield is increased by conducting deletion mutation on a coding region of the gene. Therefore, the OsCOPT7 gene can be used in rice breeding to improve the rice yield, and has a good application prospect in genetic engineering breeding.
本发明公开了铜离子转运蛋白基因OsCOPT7在水稻选育中的应用，属于植物基因工程领域。OsCOPT7基因编码蛋白质的氨基酸序列如SEQ ID NO.1所示，CDS序列如SEQ ID NO.2所示。本发明通过构建OsCOPT7基因超表达(OE)和敲除(CRISPR)转基因材料，发现OsCOPT7超表达家系单株产量下降，而OsCOPT7敲除家系产量显著提高，通过将该基因的编码区进行缺失突变提高了水稻产量。因此，OsCOPT7基因可用于水稻选育中以提高水</description><subject>AGRICULTURE</subject><subject>ANIMAL HUSBANDRY</subject><subject>BEER</subject><subject>BIOCHEMISTRY</subject><subject>CHEMISTRY</subject><subject>COMPOSITIONS THEREOF</subject><subject>CULTURE MEDIA</subject><subject>ENZYMOLOGY</subject><subject>FISHING</subject><subject>FORESTRY</subject><subject>HUMAN NECESSITIES</subject><subject>HUNTING</subject><subject>METALLURGY</subject><subject>MICROBIOLOGY</subject><subject>MICROORGANISMS OR ENZYMES</subject><subject>MUTATION OR GENETIC ENGINEERING</subject><subject>NEW PLANTS OR PROCESSES FOR OBTAINING THEM</subject><subject>ORGANIC CHEMISTRY</subject><subject>PEPTIDES</subject><subject>PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES</subject><subject>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</subject><subject>SPIRITS</subject><subject>TRAPPING</subject><subject>VINEGAR</subject><subject>WINE</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>2022</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNrjZHB2LCjIyUxOLMnMz1PIT1NIzi8oSC1SAPFKihLzigvyi0qA_PTUvFQF_2Jn_4AQc4XMPIWizORUhaSi1NSUzLx0HgbWtMSc4lReKM3NoOjmGuLsoZtakB-fWlyQmAzUXRLv7GdoaGJoaWZmbuloTIwaADGtMcY</recordid><startdate>20220318</startdate><enddate>20220318</enddate><creator>LI QIN</creator><creator>YUAN FEIRONG</creator><creator>LUO MENG</creator><creator>LYU KAI</creator><creator>LIAN XINGMING</creator><scope>EVB</scope></search><sort><creationdate>20220318</creationdate><title>Application of copper ion transporter gene OsCOPT7 in rice breeding</title><author>LI QIN ; YUAN FEIRONG ; LUO MENG ; LYU KAI ; LIAN XINGMING</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_CN114196679A3</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>chi ; eng</language><creationdate>2022</creationdate><topic>AGRICULTURE</topic><topic>ANIMAL HUSBANDRY</topic><topic>BEER</topic><topic>BIOCHEMISTRY</topic><topic>CHEMISTRY</topic><topic>COMPOSITIONS THEREOF</topic><topic>CULTURE MEDIA</topic><topic>ENZYMOLOGY</topic><topic>FISHING</topic><topic>FORESTRY</topic><topic>HUMAN NECESSITIES</topic><topic>HUNTING</topic><topic>METALLURGY</topic><topic>MICROBIOLOGY</topic><topic>MICROORGANISMS OR ENZYMES</topic><topic>MUTATION OR GENETIC ENGINEERING</topic><topic>NEW PLANTS OR PROCESSES FOR OBTAINING THEM</topic><topic>ORGANIC CHEMISTRY</topic><topic>PEPTIDES</topic><topic>PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES</topic><topic>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</topic><topic>SPIRITS</topic><topic>TRAPPING</topic><topic>VINEGAR</topic><topic>WINE</topic><toplevel>online_resources</toplevel><creatorcontrib>LI QIN</creatorcontrib><creatorcontrib>YUAN FEIRONG</creatorcontrib><creatorcontrib>LUO MENG</creatorcontrib><creatorcontrib>LYU KAI</creatorcontrib><creatorcontrib>LIAN XINGMING</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>LI QIN</au><au>YUAN FEIRONG</au><au>LUO MENG</au><au>LYU KAI</au><au>LIAN XINGMING</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>Application of copper ion transporter gene OsCOPT7 in rice breeding</title><date>2022-03-18</date><risdate>2022</risdate><abstract>The invention discloses application of a copper ion transporter gene OsCOPT7 in rice breeding, and belongs to the field of plant genetic engineering. The amino acid sequence of the protein encoded by the OsCOPT7 gene is as shown in SEQ ID NO. 1, and the CDS sequence is as shown in SEQ ID NO. 2. By constructing OsCOPT7 gene overexpression (OE) and knockout (CRISPR) transgenic materials, it is found that the yield of a single plant of an OsCOPT7 overexpression family is decreased, the yield of an OsCOPT7 knockout family is remarkably increased, and the rice yield is increased by conducting deletion mutation on a coding region of the gene. Therefore, the OsCOPT7 gene can be used in rice breeding to improve the rice yield, and has a good application prospect in genetic engineering breeding.
本发明公开了铜离子转运蛋白基因OsCOPT7在水稻选育中的应用，属于植物基因工程领域。OsCOPT7基因编码蛋白质的氨基酸序列如SEQ ID NO.1所示，CDS序列如SEQ ID NO.2所示。本发明通过构建OsCOPT7基因超表达(OE)和敲除(CRISPR)转基因材料，发现OsCOPT7超表达家系单株产量下降，而OsCOPT7敲除家系产量显著提高，通过将该基因的编码区进行缺失突变提高了水稻产量。因此，OsCOPT7基因可用于水稻选育中以提高水</abstract><oa>free_for_read</oa></addata></record> |
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| subjects | AGRICULTURE ANIMAL HUSBANDRY BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FISHING FORESTRY HUMAN NECESSITIES HUNTING METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING NEW PLANTS OR PROCESSES FOR OBTAINING THEM ORGANIC CHEMISTRY PEPTIDES PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS TRAPPING VINEGAR WINE |
| title | Application of copper ion transporter gene OsCOPT7 in rice breeding |
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