Preparation method of agmatine
The invention discloses a preparation method of agmatine, which comprises the following steps: adjusting the pH value of fermentation liquor containing arginine decarboxylase, then sequentially carrying out activated carbon decoloration and ceramic membrane filtration to obtain filtrate, carrying ou...
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| creator | CAO HUAJIE GUO YONGSHENG XIE PEI YUE MINGRUI |
| description | The invention discloses a preparation method of agmatine, which comprises the following steps: adjusting the pH value of fermentation liquor containing arginine decarboxylase, then sequentially carrying out activated carbon decoloration and ceramic membrane filtration to obtain filtrate, carrying out affinity chromatography on the filtrate by using a nickel column, then adding arginine slurry into the nickel column, then adding PLP (Poly Lactic Acid) in batches, and collecting liquid flowing out of the nickel column to obtain agmatine. The agmatine conversion liquid is obtained; performing membrane passing, first decoloration, electrodialysis, first concentration and first separation on the agmatine conversion liquid to obtain a agmatine crude product; re-dissolving the crude product, decolorizing for the second time, concentrating for the second time, stirring for growing crystal, and separating for the second time to obtain a gamatine refined product; and finally drying to obtain solid gamatine. The prepara |
| format | Patent |
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The agmatine conversion liquid is obtained; performing membrane passing, first decoloration, electrodialysis, first concentration and first separation on the agmatine conversion liquid to obtain a agmatine crude product; re-dissolving the crude product, decolorizing for the second time, concentrating for the second time, stirring for growing crystal, and separating for the second time to obtain a gamatine refined product; and finally drying to obtain solid gamatine. 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The agmatine conversion liquid is obtained; performing membrane passing, first decoloration, electrodialysis, first concentration and first separation on the agmatine conversion liquid to obtain a agmatine crude product; re-dissolving the crude product, decolorizing for the second time, concentrating for the second time, stirring for growing crystal, and separating for the second time to obtain a gamatine refined product; and finally drying to obtain solid gamatine. 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The agmatine conversion liquid is obtained; performing membrane passing, first decoloration, electrodialysis, first concentration and first separation on the agmatine conversion liquid to obtain a agmatine crude product; re-dissolving the crude product, decolorizing for the second time, concentrating for the second time, stirring for growing crystal, and separating for the second time to obtain a gamatine refined product; and finally drying to obtain solid gamatine. The prepara</abstract><oa>free_for_read</oa></addata></record> |
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| subjects | ACYCLIC OR CARBOCYCLIC COMPOUNDS BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING ORGANIC CHEMISTRY PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE |
| title | Preparation method of agmatine |
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