Carbon coupling composite microbial inoculum and preparation method and application thereof
The invention relates to the technical field of biomass resource utilization and environmental pollutant treatment, in particular to a preparation method of a carbon coupling composite microbial inoculum. The method comprises the following steps of: S1, treating a natural material capable of being c...
Gespeichert in:
| Hauptverfasser: | , , , , , , , , , |
|---|---|
| Format: | Patent |
| Sprache: | chi ; eng |
| Schlagworte: | |
| Online-Zugang: | Volltext bestellen |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | |
|---|---|
| container_issue | |
| container_start_page | |
| container_title | |
| container_volume | |
| creator | LIN JINHAO QIU ZHISHEN WANG LING YU WANXUE BU SHAOTENG LI YANLIANG LAN SHANHONG LIU MEIJIE HUANG JIANZHI LI LEI |
| description | The invention relates to the technical field of biomass resource utilization and environmental pollutant treatment, in particular to a preparation method of a carbon coupling composite microbial inoculum. The method comprises the following steps of: S1, treating a natural material capable of being carbonized; S2, collecting a soil sample of typical polluted point soil, and separating and culturing a target strain through an in-situ culture method; S3, performing domesticating to obtain a strain capable of degrading a target pollutant under the stress of heavy metal; S4, conducting separating and purifying to obtain a pure bacterial colony of the degrading bacteria; S5, repeating the S4, collecting at least three thalli, performing enrichment culture until OD600 is equal to 1.0-1.2, collecting bacterial liquid, and performing cryopreservation; S6, mixing various bacterial liquid to obtain a compound bacterial seed solution; and S7, performing mixed culture on the compound bacterial seed solution and a carbon-b |
| format | Patent |
| fullrecord | <record><control><sourceid>epo_EVB</sourceid><recordid>TN_cdi_epo_espacenet_CN114058507A</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>CN114058507A</sourcerecordid><originalsourceid>FETCH-epo_espacenet_CN114058507A3</originalsourceid><addsrcrecordid>eNqNiz0KAjEQRtNYiHqHeABhF120laBYWdlZLLPZWXcgyQz5ub9BPYDV9_F4b6meBuLAQVsu4ii86vHCiTJqTzbyQOA0BbbFFa8hjFoiCkTIVCuPeebxg0Fqb784zxiRp7VaTOASbn67Utvr5WFuOxTuMQlYDJh7c2_bQ9OduuZ43v_jvAHTnTw0</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>patent</recordtype></control><display><type>patent</type><title>Carbon coupling composite microbial inoculum and preparation method and application thereof</title><source>esp@cenet</source><creator>LIN JINHAO ; QIU ZHISHEN ; WANG LING ; YU WANXUE ; BU SHAOTENG ; LI YANLIANG ; LAN SHANHONG ; LIU MEIJIE ; HUANG JIANZHI ; LI LEI</creator><creatorcontrib>LIN JINHAO ; QIU ZHISHEN ; WANG LING ; YU WANXUE ; BU SHAOTENG ; LI YANLIANG ; LAN SHANHONG ; LIU MEIJIE ; HUANG JIANZHI ; LI LEI</creatorcontrib><description>The invention relates to the technical field of biomass resource utilization and environmental pollutant treatment, in particular to a preparation method of a carbon coupling composite microbial inoculum. The method comprises the following steps of: S1, treating a natural material capable of being carbonized; S2, collecting a soil sample of typical polluted point soil, and separating and culturing a target strain through an in-situ culture method; S3, performing domesticating to obtain a strain capable of degrading a target pollutant under the stress of heavy metal; S4, conducting separating and purifying to obtain a pure bacterial colony of the degrading bacteria; S5, repeating the S4, collecting at least three thalli, performing enrichment culture until OD600 is equal to 1.0-1.2, collecting bacterial liquid, and performing cryopreservation; S6, mixing various bacterial liquid to obtain a compound bacterial seed solution; and S7, performing mixed culture on the compound bacterial seed solution and a carbon-b</description><language>chi ; eng</language><subject>BEER ; BIOCHEMISTRY ; CHEMISTRY ; COMPOSITIONS THEREOF ; CULTURE MEDIA ; DISPOSAL OF SOLID WASTE ; ENZYMOLOGY ; METALLURGY ; MICROBIOLOGY ; MICROORGANISMS OR ENZYMES ; MUTATION OR GENETIC ENGINEERING ; PERFORMING OPERATIONS ; PROCESSES USING MICROORGANISMS ; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS ; RECLAMATION OF CONTAMINATED SOIL ; RECLAMATION OF CONTAMINED SOIL SOIL ; SPIRITS ; TRANSPORTING ; VINEGAR ; WINE</subject><creationdate>2022</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20220218&DB=EPODOC&CC=CN&NR=114058507A$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,780,885,25564,76547</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20220218&DB=EPODOC&CC=CN&NR=114058507A$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>LIN JINHAO</creatorcontrib><creatorcontrib>QIU ZHISHEN</creatorcontrib><creatorcontrib>WANG LING</creatorcontrib><creatorcontrib>YU WANXUE</creatorcontrib><creatorcontrib>BU SHAOTENG</creatorcontrib><creatorcontrib>LI YANLIANG</creatorcontrib><creatorcontrib>LAN SHANHONG</creatorcontrib><creatorcontrib>LIU MEIJIE</creatorcontrib><creatorcontrib>HUANG JIANZHI</creatorcontrib><creatorcontrib>LI LEI</creatorcontrib><title>Carbon coupling composite microbial inoculum and preparation method and application thereof</title><description>The invention relates to the technical field of biomass resource utilization and environmental pollutant treatment, in particular to a preparation method of a carbon coupling composite microbial inoculum. The method comprises the following steps of: S1, treating a natural material capable of being carbonized; S2, collecting a soil sample of typical polluted point soil, and separating and culturing a target strain through an in-situ culture method; S3, performing domesticating to obtain a strain capable of degrading a target pollutant under the stress of heavy metal; S4, conducting separating and purifying to obtain a pure bacterial colony of the degrading bacteria; S5, repeating the S4, collecting at least three thalli, performing enrichment culture until OD600 is equal to 1.0-1.2, collecting bacterial liquid, and performing cryopreservation; S6, mixing various bacterial liquid to obtain a compound bacterial seed solution; and S7, performing mixed culture on the compound bacterial seed solution and a carbon-b</description><subject>BEER</subject><subject>BIOCHEMISTRY</subject><subject>CHEMISTRY</subject><subject>COMPOSITIONS THEREOF</subject><subject>CULTURE MEDIA</subject><subject>DISPOSAL OF SOLID WASTE</subject><subject>ENZYMOLOGY</subject><subject>METALLURGY</subject><subject>MICROBIOLOGY</subject><subject>MICROORGANISMS OR ENZYMES</subject><subject>MUTATION OR GENETIC ENGINEERING</subject><subject>PERFORMING OPERATIONS</subject><subject>PROCESSES USING MICROORGANISMS</subject><subject>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</subject><subject>RECLAMATION OF CONTAMINATED SOIL</subject><subject>RECLAMATION OF CONTAMINED SOIL SOIL</subject><subject>SPIRITS</subject><subject>TRANSPORTING</subject><subject>VINEGAR</subject><subject>WINE</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>2022</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNqNiz0KAjEQRtNYiHqHeABhF120laBYWdlZLLPZWXcgyQz5ub9BPYDV9_F4b6meBuLAQVsu4ii86vHCiTJqTzbyQOA0BbbFFa8hjFoiCkTIVCuPeebxg0Fqb784zxiRp7VaTOASbn67Utvr5WFuOxTuMQlYDJh7c2_bQ9OduuZ43v_jvAHTnTw0</recordid><startdate>20220218</startdate><enddate>20220218</enddate><creator>LIN JINHAO</creator><creator>QIU ZHISHEN</creator><creator>WANG LING</creator><creator>YU WANXUE</creator><creator>BU SHAOTENG</creator><creator>LI YANLIANG</creator><creator>LAN SHANHONG</creator><creator>LIU MEIJIE</creator><creator>HUANG JIANZHI</creator><creator>LI LEI</creator><scope>EVB</scope></search><sort><creationdate>20220218</creationdate><title>Carbon coupling composite microbial inoculum and preparation method and application thereof</title><author>LIN JINHAO ; QIU ZHISHEN ; WANG LING ; YU WANXUE ; BU SHAOTENG ; LI YANLIANG ; LAN SHANHONG ; LIU MEIJIE ; HUANG JIANZHI ; LI LEI</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_CN114058507A3</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>chi ; eng</language><creationdate>2022</creationdate><topic>BEER</topic><topic>BIOCHEMISTRY</topic><topic>CHEMISTRY</topic><topic>COMPOSITIONS THEREOF</topic><topic>CULTURE MEDIA</topic><topic>DISPOSAL OF SOLID WASTE</topic><topic>ENZYMOLOGY</topic><topic>METALLURGY</topic><topic>MICROBIOLOGY</topic><topic>MICROORGANISMS OR ENZYMES</topic><topic>MUTATION OR GENETIC ENGINEERING</topic><topic>PERFORMING OPERATIONS</topic><topic>PROCESSES USING MICROORGANISMS</topic><topic>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</topic><topic>RECLAMATION OF CONTAMINATED SOIL</topic><topic>RECLAMATION OF CONTAMINED SOIL SOIL</topic><topic>SPIRITS</topic><topic>TRANSPORTING</topic><topic>VINEGAR</topic><topic>WINE</topic><toplevel>online_resources</toplevel><creatorcontrib>LIN JINHAO</creatorcontrib><creatorcontrib>QIU ZHISHEN</creatorcontrib><creatorcontrib>WANG LING</creatorcontrib><creatorcontrib>YU WANXUE</creatorcontrib><creatorcontrib>BU SHAOTENG</creatorcontrib><creatorcontrib>LI YANLIANG</creatorcontrib><creatorcontrib>LAN SHANHONG</creatorcontrib><creatorcontrib>LIU MEIJIE</creatorcontrib><creatorcontrib>HUANG JIANZHI</creatorcontrib><creatorcontrib>LI LEI</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>LIN JINHAO</au><au>QIU ZHISHEN</au><au>WANG LING</au><au>YU WANXUE</au><au>BU SHAOTENG</au><au>LI YANLIANG</au><au>LAN SHANHONG</au><au>LIU MEIJIE</au><au>HUANG JIANZHI</au><au>LI LEI</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>Carbon coupling composite microbial inoculum and preparation method and application thereof</title><date>2022-02-18</date><risdate>2022</risdate><abstract>The invention relates to the technical field of biomass resource utilization and environmental pollutant treatment, in particular to a preparation method of a carbon coupling composite microbial inoculum. The method comprises the following steps of: S1, treating a natural material capable of being carbonized; S2, collecting a soil sample of typical polluted point soil, and separating and culturing a target strain through an in-situ culture method; S3, performing domesticating to obtain a strain capable of degrading a target pollutant under the stress of heavy metal; S4, conducting separating and purifying to obtain a pure bacterial colony of the degrading bacteria; S5, repeating the S4, collecting at least three thalli, performing enrichment culture until OD600 is equal to 1.0-1.2, collecting bacterial liquid, and performing cryopreservation; S6, mixing various bacterial liquid to obtain a compound bacterial seed solution; and S7, performing mixed culture on the compound bacterial seed solution and a carbon-b</abstract><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext_linktorsrc |
| identifier | |
| ispartof | |
| issn | |
| language | chi ; eng |
| recordid | cdi_epo_espacenet_CN114058507A |
| source | esp@cenet |
| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA DISPOSAL OF SOLID WASTE ENZYMOLOGY METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PERFORMING OPERATIONS PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS RECLAMATION OF CONTAMINATED SOIL RECLAMATION OF CONTAMINED SOIL SOIL SPIRITS TRANSPORTING VINEGAR WINE |
| title | Carbon coupling composite microbial inoculum and preparation method and application thereof |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-27T05%3A09%3A47IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-epo_EVB&rft_val_fmt=info:ofi/fmt:kev:mtx:patent&rft.genre=patent&rft.au=LIN%20JINHAO&rft.date=2022-02-18&rft_id=info:doi/&rft_dat=%3Cepo_EVB%3ECN114058507A%3C/epo_EVB%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_id=info:pmid/&rfr_iscdi=true |