Oligo-dT affinity chromatography filler
The invention provides an Oligo-dT affinity chromatography filler which is prepared by taking a polymer microsphere with a hydrophilic surface and polyhydroxy hydrophilic functional groups as a base sphere and bonding a dT nucleotide monomer through a hydroxyl group on the surface of the polymer mic...
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| creator | LIU JINGSONG QI ZIYAN JIN BAISHENG WANG JIE LIN SHENGYUE ZHAO GUANGYAO LIU GANG JIANG BIWANG |
| description | The invention provides an Oligo-dT affinity chromatography filler which is prepared by taking a polymer microsphere with a hydrophilic surface and polyhydroxy hydrophilic functional groups as a base sphere and bonding a dT nucleotide monomer through a hydroxyl group on the surface of the polymer microsphere to generate an oligo-dT ligand on the surface of a polymer. Compared with the conventional Oligo-dT affinity filler, the Oligo-dT affinity filler disclosed by the invention has higher mRNA loading capacity and higher mRNA recovery rate, and also has better alkali resistance than the conventional Oligo-dT affinity filler. The preparation method is simple, the synthesis and purification of an oligo-dT ligand required by the production of the oligo-dT affinity filler in the prior art are not needed, and the production cost of the affinity filler can be greatly reduced, so that the production and purification cost of mRNA is reduced.
本发明提供了一种Oligo-dT亲和层析填料，以表面亲水的、具有多羟基亲水官能团的聚合物微球为基球，通过聚合物微球表面的羟基将dT核苷酸单体键合生成oli |
| format | Patent |
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本发明提供了一种Oligo-dT亲和层析填料，以表面亲水的、具有多羟基亲水官能团的聚合物微球为基球，通过聚合物微球表面的羟基将dT核苷酸单体键合生成oli</description><language>chi ; eng</language><subject>CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOIDCHEMISTRY ; PERFORMING OPERATIONS ; PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL ; THEIR RELEVANT APPARATUS ; TRANSPORTING</subject><creationdate>2022</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20220218&DB=EPODOC&CC=CN&NR=114054006A$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,780,885,25564,76547</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20220218&DB=EPODOC&CC=CN&NR=114054006A$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>LIU JINGSONG</creatorcontrib><creatorcontrib>QI ZIYAN</creatorcontrib><creatorcontrib>JIN BAISHENG</creatorcontrib><creatorcontrib>WANG JIE</creatorcontrib><creatorcontrib>LIN SHENGYUE</creatorcontrib><creatorcontrib>ZHAO GUANGYAO</creatorcontrib><creatorcontrib>LIU GANG</creatorcontrib><creatorcontrib>JIANG BIWANG</creatorcontrib><title>Oligo-dT affinity chromatography filler</title><description>The invention provides an Oligo-dT affinity chromatography filler which is prepared by taking a polymer microsphere with a hydrophilic surface and polyhydroxy hydrophilic functional groups as a base sphere and bonding a dT nucleotide monomer through a hydroxyl group on the surface of the polymer microsphere to generate an oligo-dT ligand on the surface of a polymer. Compared with the conventional Oligo-dT affinity filler, the Oligo-dT affinity filler disclosed by the invention has higher mRNA loading capacity and higher mRNA recovery rate, and also has better alkali resistance than the conventional Oligo-dT affinity filler. The preparation method is simple, the synthesis and purification of an oligo-dT ligand required by the production of the oligo-dT affinity filler in the prior art are not needed, and the production cost of the affinity filler can be greatly reduced, so that the production and purification cost of mRNA is reduced.
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本发明提供了一种Oligo-dT亲和层析填料，以表面亲水的、具有多羟基亲水官能团的聚合物微球为基球，通过聚合物微球表面的羟基将dT核苷酸单体键合生成oli</abstract><oa>free_for_read</oa></addata></record> |
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| subjects | CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOIDCHEMISTRY PERFORMING OPERATIONS PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL THEIR RELEVANT APPARATUS TRANSPORTING |
| title | Oligo-dT affinity chromatography filler |
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