Arginine deiminase producing strain and construction method thereof
The invention discloses an arginine deiminase producing strain and a construction method thereof. The method comprises the following steps: carrying out double enzyme digestion treatment on a plasmid pWB980 by using EcoR I and KpnI, integrating a Pg3 strong promoter sequence onto the plasmid pWB980...
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| creator | CAO HUAJIE GUO YONGSHENG XIE PEI YUE MINGRUI |
| description | The invention discloses an arginine deiminase producing strain and a construction method thereof. The method comprises the following steps: carrying out double enzyme digestion treatment on a plasmid pWB980 by using EcoR I and KpnI, integrating a Pg3 strong promoter sequence onto the plasmid pWB980 subjected to the double enzyme digestion treatment to obtain a plasmid pWB980-Pg3, carrying out enzyme digestion treatment on the plasmid pWB980-Pg3 by using BamH I and SphI, and integrating an arcA gene onto the plasmid pWB980-Pg3 subjected to the enzyme digestion treatment to obtain a recombinant expression vector; and introducing the obtained recombinant expression vector into bacillus subtilis, and constructing to obtain the arginine deiminase producing strain. According to the invention, arginine deiminase is prepared by fermentation of the producing strain provided by the invention, and the enzyme activity of the arginine deiminase can be remarkably improved.
本发明公开了一种精氨酸脱亚胺酶生产菌及其构建方法。其将质粒pWB980用EcoRⅠ和KpnⅠ双酶切处 |
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本发明公开了一种精氨酸脱亚胺酶生产菌及其构建方法。其将质粒pWB980用EcoRⅠ和KpnⅠ双酶切处</description><language>chi ; eng</language><subject>BEER ; BIOCHEMISTRY ; CHEMISTRY ; COMPOSITIONS THEREOF ; CULTURE MEDIA ; ENZYMOLOGY ; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE ; METALLURGY ; MICROBIOLOGY ; MICROORGANISMS OR ENZYMES ; MUTATION OR GENETIC ENGINEERING ; PROCESSES USING MICROORGANISMS ; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS ; SPIRITS ; VINEGAR ; WINE</subject><creationdate>2022</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20220201&DB=EPODOC&CC=CN&NR=113999834A$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,776,881,25544,76293</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20220201&DB=EPODOC&CC=CN&NR=113999834A$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>CAO HUAJIE</creatorcontrib><creatorcontrib>GUO YONGSHENG</creatorcontrib><creatorcontrib>XIE PEI</creatorcontrib><creatorcontrib>YUE MINGRUI</creatorcontrib><title>Arginine deiminase producing strain and construction method thereof</title><description>The invention discloses an arginine deiminase producing strain and a construction method thereof. The method comprises the following steps: carrying out double enzyme digestion treatment on a plasmid pWB980 by using EcoR I and KpnI, integrating a Pg3 strong promoter sequence onto the plasmid pWB980 subjected to the double enzyme digestion treatment to obtain a plasmid pWB980-Pg3, carrying out enzyme digestion treatment on the plasmid pWB980-Pg3 by using BamH I and SphI, and integrating an arcA gene onto the plasmid pWB980-Pg3 subjected to the enzyme digestion treatment to obtain a recombinant expression vector; and introducing the obtained recombinant expression vector into bacillus subtilis, and constructing to obtain the arginine deiminase producing strain. According to the invention, arginine deiminase is prepared by fermentation of the producing strain provided by the invention, and the enzyme activity of the arginine deiminase can be remarkably improved.
本发明公开了一种精氨酸脱亚胺酶生产菌及其构建方法。其将质粒pWB980用EcoRⅠ和KpnⅠ双酶切处</description><subject>BEER</subject><subject>BIOCHEMISTRY</subject><subject>CHEMISTRY</subject><subject>COMPOSITIONS THEREOF</subject><subject>CULTURE MEDIA</subject><subject>ENZYMOLOGY</subject><subject>FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE</subject><subject>METALLURGY</subject><subject>MICROBIOLOGY</subject><subject>MICROORGANISMS OR ENZYMES</subject><subject>MUTATION OR GENETIC ENGINEERING</subject><subject>PROCESSES USING MICROORGANISMS</subject><subject>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</subject><subject>SPIRITS</subject><subject>VINEGAR</subject><subject>WINE</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>2022</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNqNyrEKwjAURuEsDqK-w_UBHEoczFhCxcnJvYTkb3vB3huS9P118AGcDh-cvfF9mVlYQAm8soQKykXTFllmqq0EFgqSKKp8tcXGKrSiLZqoLSjQ6Wh2U3hXnH49mPN9ePnHBVlH1BwiBG30z66zzrmbvfb2n-cDxsAzLA</recordid><startdate>20220201</startdate><enddate>20220201</enddate><creator>CAO HUAJIE</creator><creator>GUO YONGSHENG</creator><creator>XIE PEI</creator><creator>YUE MINGRUI</creator><scope>EVB</scope></search><sort><creationdate>20220201</creationdate><title>Arginine deiminase producing strain and construction method thereof</title><author>CAO HUAJIE ; GUO YONGSHENG ; XIE PEI ; YUE MINGRUI</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_CN113999834A3</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>chi ; eng</language><creationdate>2022</creationdate><topic>BEER</topic><topic>BIOCHEMISTRY</topic><topic>CHEMISTRY</topic><topic>COMPOSITIONS THEREOF</topic><topic>CULTURE MEDIA</topic><topic>ENZYMOLOGY</topic><topic>FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE</topic><topic>METALLURGY</topic><topic>MICROBIOLOGY</topic><topic>MICROORGANISMS OR ENZYMES</topic><topic>MUTATION OR GENETIC ENGINEERING</topic><topic>PROCESSES USING MICROORGANISMS</topic><topic>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</topic><topic>SPIRITS</topic><topic>VINEGAR</topic><topic>WINE</topic><toplevel>online_resources</toplevel><creatorcontrib>CAO HUAJIE</creatorcontrib><creatorcontrib>GUO YONGSHENG</creatorcontrib><creatorcontrib>XIE PEI</creatorcontrib><creatorcontrib>YUE MINGRUI</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>CAO HUAJIE</au><au>GUO YONGSHENG</au><au>XIE PEI</au><au>YUE MINGRUI</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>Arginine deiminase producing strain and construction method thereof</title><date>2022-02-01</date><risdate>2022</risdate><abstract>The invention discloses an arginine deiminase producing strain and a construction method thereof. The method comprises the following steps: carrying out double enzyme digestion treatment on a plasmid pWB980 by using EcoR I and KpnI, integrating a Pg3 strong promoter sequence onto the plasmid pWB980 subjected to the double enzyme digestion treatment to obtain a plasmid pWB980-Pg3, carrying out enzyme digestion treatment on the plasmid pWB980-Pg3 by using BamH I and SphI, and integrating an arcA gene onto the plasmid pWB980-Pg3 subjected to the enzyme digestion treatment to obtain a recombinant expression vector; and introducing the obtained recombinant expression vector into bacillus subtilis, and constructing to obtain the arginine deiminase producing strain. According to the invention, arginine deiminase is prepared by fermentation of the producing strain provided by the invention, and the enzyme activity of the arginine deiminase can be remarkably improved.
本发明公开了一种精氨酸脱亚胺酶生产菌及其构建方法。其将质粒pWB980用EcoRⅠ和KpnⅠ双酶切处</abstract><oa>free_for_read</oa></addata></record> |
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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE |
| title | Arginine deiminase producing strain and construction method thereof |
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