High-efficiency expression method of human myoglobin
The invention discloses a high-efficiency expression method of human myoglobin, and particularly relates to recombinant human myoglobin which is high in expression quantity, soluble in expression and excellent in activity is obtained by performing codon optimization on a base sequence of the myoglob...
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| creator | YAN QINGQING WANG YUTAI KUANG XIULI HUANG HONG JIANG XIWEN |
| description | The invention discloses a high-efficiency expression method of human myoglobin, and particularly relates to recombinant human myoglobin which is high in expression quantity, soluble in expression and excellent in activity is obtained by performing codon optimization on a base sequence of the myoglobin. Besides, the recombinant myoglobin expressed by the invention only has a (His) 6 label at the C terminal, the purification step is simplified, the recovery rate and the purity are improved, the high-activity recombinant myoglobin can be obtained, a diagnostic reagent raw material is provided for clinical detection of myocardial injury and prognosis, and a foundation is laid for preparation of a rapid diagnostic kit for heart diseases such as acute myocardial infarction.
本发明公开了人肌红蛋白的高效表达方法，具体地本发明通过对肌红蛋白的碱基序列进行密码子优化，获得了表达量高、可溶性表达且保持优异活性的重组人肌红蛋白。此外，本发明表达的重组肌红蛋白只有C端有(His)6标签，简化了纯化步骤，提高了回收率及纯度，能够获得高活性的重组肌红蛋白，为临床检测心肌损伤及预后提供诊断试剂原料，为急性心肌梗死等心脏疾病的快速诊断试剂盒的制备奠定了基础。 |
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本发明公开了人肌红蛋白的高效表达方法，具体地本发明通过对肌红蛋白的碱基序列进行密码子优化，获得了表达量高、可溶性表达且保持优异活性的重组人肌红蛋白。此外，本发明表达的重组肌红蛋白只有C端有(His)6标签，简化了纯化步骤，提高了回收率及纯度，能够获得高活性的重组肌红蛋白，为临床检测心肌损伤及预后提供诊断试剂原料，为急性心肌梗死等心脏疾病的快速诊断试剂盒的制备奠定了基础。</description><language>chi ; eng</language><subject>BEER ; BIOCHEMISTRY ; CHEMISTRY ; COMPOSITIONS THEREOF ; CULTURE MEDIA ; ENZYMOLOGY ; METALLURGY ; MICROBIOLOGY ; MICROORGANISMS OR ENZYMES ; MUTATION OR GENETIC ENGINEERING ; ORGANIC CHEMISTRY ; PEPTIDES ; PROCESSES USING MICROORGANISMS ; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS ; SPIRITS ; VINEGAR ; WINE</subject><creationdate>2022</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20220201&DB=EPODOC&CC=CN&NR=113999299A$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,776,881,25542,76290</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20220201&DB=EPODOC&CC=CN&NR=113999299A$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>YAN QINGQING</creatorcontrib><creatorcontrib>WANG YUTAI</creatorcontrib><creatorcontrib>KUANG XIULI</creatorcontrib><creatorcontrib>HUANG HONG</creatorcontrib><creatorcontrib>JIANG XIWEN</creatorcontrib><title>High-efficiency expression method of human myoglobin</title><description>The invention discloses a high-efficiency expression method of human myoglobin, and particularly relates to recombinant human myoglobin which is high in expression quantity, soluble in expression and excellent in activity is obtained by performing codon optimization on a base sequence of the myoglobin. Besides, the recombinant myoglobin expressed by the invention only has a (His) 6 label at the C terminal, the purification step is simplified, the recovery rate and the purity are improved, the high-activity recombinant myoglobin can be obtained, a diagnostic reagent raw material is provided for clinical detection of myocardial injury and prognosis, and a foundation is laid for preparation of a rapid diagnostic kit for heart diseases such as acute myocardial infarction.
本发明公开了人肌红蛋白的高效表达方法，具体地本发明通过对肌红蛋白的碱基序列进行密码子优化，获得了表达量高、可溶性表达且保持优异活性的重组人肌红蛋白。此外，本发明表达的重组肌红蛋白只有C端有(His)6标签，简化了纯化步骤，提高了回收率及纯度，能够获得高活性的重组肌红蛋白，为临床检测心肌损伤及预后提供诊断试剂原料，为急性心肌梗死等心脏疾病的快速诊断试剂盒的制备奠定了基础。</description><subject>BEER</subject><subject>BIOCHEMISTRY</subject><subject>CHEMISTRY</subject><subject>COMPOSITIONS THEREOF</subject><subject>CULTURE MEDIA</subject><subject>ENZYMOLOGY</subject><subject>METALLURGY</subject><subject>MICROBIOLOGY</subject><subject>MICROORGANISMS OR ENZYMES</subject><subject>MUTATION OR GENETIC ENGINEERING</subject><subject>ORGANIC CHEMISTRY</subject><subject>PEPTIDES</subject><subject>PROCESSES USING MICROORGANISMS</subject><subject>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</subject><subject>SPIRITS</subject><subject>VINEGAR</subject><subject>WINE</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>2022</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNrjZDDxyEzP0E1NS8tMzkzNS65USK0oKEotLs7Mz1PITS3JyE9RyE9TyCjNTQTyK_PTc_KTMvN4GFjTEnOKU3mhNDeDoptriLOHbmpBfnxqcUFicmpeakm8s5-hobGlpaWRpaWjMTFqAFBPLTQ</recordid><startdate>20220201</startdate><enddate>20220201</enddate><creator>YAN QINGQING</creator><creator>WANG YUTAI</creator><creator>KUANG XIULI</creator><creator>HUANG HONG</creator><creator>JIANG XIWEN</creator><scope>EVB</scope></search><sort><creationdate>20220201</creationdate><title>High-efficiency expression method of human myoglobin</title><author>YAN QINGQING ; WANG YUTAI ; KUANG XIULI ; HUANG HONG ; JIANG XIWEN</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_CN113999299A3</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>chi ; eng</language><creationdate>2022</creationdate><topic>BEER</topic><topic>BIOCHEMISTRY</topic><topic>CHEMISTRY</topic><topic>COMPOSITIONS THEREOF</topic><topic>CULTURE MEDIA</topic><topic>ENZYMOLOGY</topic><topic>METALLURGY</topic><topic>MICROBIOLOGY</topic><topic>MICROORGANISMS OR ENZYMES</topic><topic>MUTATION OR GENETIC ENGINEERING</topic><topic>ORGANIC CHEMISTRY</topic><topic>PEPTIDES</topic><topic>PROCESSES USING MICROORGANISMS</topic><topic>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</topic><topic>SPIRITS</topic><topic>VINEGAR</topic><topic>WINE</topic><toplevel>online_resources</toplevel><creatorcontrib>YAN QINGQING</creatorcontrib><creatorcontrib>WANG YUTAI</creatorcontrib><creatorcontrib>KUANG XIULI</creatorcontrib><creatorcontrib>HUANG HONG</creatorcontrib><creatorcontrib>JIANG XIWEN</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>YAN QINGQING</au><au>WANG YUTAI</au><au>KUANG XIULI</au><au>HUANG HONG</au><au>JIANG XIWEN</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>High-efficiency expression method of human myoglobin</title><date>2022-02-01</date><risdate>2022</risdate><abstract>The invention discloses a high-efficiency expression method of human myoglobin, and particularly relates to recombinant human myoglobin which is high in expression quantity, soluble in expression and excellent in activity is obtained by performing codon optimization on a base sequence of the myoglobin. Besides, the recombinant myoglobin expressed by the invention only has a (His) 6 label at the C terminal, the purification step is simplified, the recovery rate and the purity are improved, the high-activity recombinant myoglobin can be obtained, a diagnostic reagent raw material is provided for clinical detection of myocardial injury and prognosis, and a foundation is laid for preparation of a rapid diagnostic kit for heart diseases such as acute myocardial infarction.
本发明公开了人肌红蛋白的高效表达方法，具体地本发明通过对肌红蛋白的碱基序列进行密码子优化，获得了表达量高、可溶性表达且保持优异活性的重组人肌红蛋白。此外，本发明表达的重组肌红蛋白只有C端有(His)6标签，简化了纯化步骤，提高了回收率及纯度，能够获得高活性的重组肌红蛋白，为临床检测心肌损伤及预后提供诊断试剂原料，为急性心肌梗死等心脏疾病的快速诊断试剂盒的制备奠定了基础。</abstract><oa>free_for_read</oa></addata></record> |
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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING ORGANIC CHEMISTRY PEPTIDES PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE |
| title | High-efficiency expression method of human myoglobin |
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