Nucleic acid isothermal amplification detection reagent, kit, detection system and method

The invention discloses a nucleic acid isothermal amplification detection reagent, a kit, a detection system and a method, the reagent is in a freeze-dried powder state and comprises a buffer solution, MgCl2, dATP, dGTP, dCTP, dTTP, dUTP, UNG enzyme, Bst DNA polymerase, reverse transcriptase, trehalose, sucrose, mannitol, Tween20, guanidine hydrochloride and a primer composition of LAMP of a virus to be detected. A system suitable for direct amplification of a sample is adopted, pathogens can be split, genome nucleic acid of the pathogens can be released, an inhibition-resistant buffer system is provided, the nucleic acid extraction-free effect is achieved, meanwhile, normal-temperature storage of the freeze-dried reagent is achieved, the cold-chain transportation defect is reduced. According to the detection system provided by the invention, the detection reagent is combined with the visual nucleic acid isothermal amplification device detection equipment with the reflecting mirror, so that sample feeding and