Lentivirus dissolution buffer solution and application thereof

The invention discloses a lentivirus dissolution buffer solution and application thereof. The lentivirus dissolution buffer solution comprises 3-10 mM of Tris, 15-35 mM of proline, 1-5 mM of MgCl2, 5-20% of lactose, 0.005-0.02% of Tween 80 and the balance of water, the percentage is mass percent, an...

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Hauptverfasser: SUI LILI, HU DICHAO, KONG LINGJIE, PI CHUANZHEN
Format: Patent
Sprache:chi ; eng
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Zusammenfassung:The invention discloses a lentivirus dissolution buffer solution and application thereof. The lentivirus dissolution buffer solution comprises 3-10 mM of Tris, 15-35 mM of proline, 1-5 mM of MgCl2, 5-20% of lactose, 0.005-0.02% of Tween 80 and the balance of water, the percentage is mass percent, and the pH value of the lentivirus dissolution buffer solution is 7-9. The components of the lentivirus dissolution buffer solution are all pharmaceutic adjuvants, the influence on the lentivirus activity can be avoided, and the virus structure can be stabilized and the virus activity can be enhanced by adding proline into the lentivirus dissolution buffer solution; the Tween80 can be used for preventing protein or virus aggregation; and MgCl2 has the effect of maintaining protein or virus activity, through the combined effect of several components in the formula, the activity and recovery rate of lentivirus during purification can be comprehensively improved, and the transduction efficiency of lentivirus and the pos