Method for determining related substances of propofol tenofovir by high performance liquid chromatography

The invention provides a method for determining related substances of propofol tenofovir by high performance liquid chromatography, and the high performance liquid chromatography conditions are as follows: a C18 silica gel chromatographic column is used, the detection wavelength is 260 nm, the flow velocity is 1.0 ml/min, the column temperature is 25-30 DEG C, the sample size is 10-50 [mu] l, the mobile phase is composed of a phase A and a phase B, wherein the volume ratio of a weakly acidic buffer salt solution to an organic solvent in the phase A is (80.0-99.9): 1, and the volume ratio of the weakly acidic buffer salt solution to the organic solvent in the phase B is (40-60): (60-40), and gradient elution is carried out. According to the method, a C18 silica gel column with high universality is selected to perform high performance liquid chromatography detection on the related substances of the propofol tenofovir, elution conditions are optimized, various related substances can be detected, and the method h