Construction method of duck ACE2 gene eukaryotic expression plasmid and application of duck ACE2 gene eukaryotic expression plasmid in construction of CHO stable cell lines

The invention relates to construction and identification of a recombinant plasmid pcDNA3.1-ACE2 capable of expressing exogenous duck ACE2, and determination of screening concentration of CHO cells G418 with stress. The recombinant plasmid is transfected into CHO through stable transfection, and recombinant CHO cell lines capable of expressing exogenous ACE2 protein is constructed by means of immunofluorescence and Western-blot technology under screening of G418 with stress. 本发明涉及可以表达外源鸭ACE2的重组质粒pcDNA3.1-ACE2的构建以及鉴定，以及CHO细胞G418压力筛选浓度的确定，通过稳定转染将该重组质粒转染进CHO内，在G418压力筛选下，借助免疫荧光及Western-blot技术构建了可以表达外源ACE2蛋白的重组CHO细胞系。