Primer group, kit and method for detecting Chinese type G gamma+(A gamma delta beta) thalassemia deletion variation
The invention provides a primer group, a kit and a method for detecting Chinese type G gamma+(A gamma delta beta) thalassemia deletion variation on the basis of a long-fragment PCR (Polymerase Chain Reaction) amplification and third-generation high-throughput sequencing technology platform. The prim...
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| creator | CHEN YUJUN GUI BAOHENG MAO AIPING GUI CHUNRONG HUANG YAN XIE BOBO LAI YINGHUI WEI XIANDA LIU JULIANG WEI WEI |
| description | The invention provides a primer group, a kit and a method for detecting Chinese type G gamma+(A gamma delta beta) thalassemia deletion variation on the basis of a long-fragment PCR (Polymerase Chain Reaction) amplification and third-generation high-throughput sequencing technology platform. The primer group, the kit and the method for detecting the Chinese type G gamma+(A gamma delta beta) thalassemia deletion variation aim to solve the problems that Gap-PCR and MLPA technologies conventionally adopted in laboratories at present cannot accurately detect fracture sites, the throughput is low, the cost is high, the operation is tedious, the reading length of second-generation high-throughput sequencing is long, and complex structure variation of thalassemia gene clusters cannot be detected. According to the primer group, the kit and the method for detecting the Chinese type G gamma+(A gamma delta beta) thalassemia deletion variation, accurate and rapid detection of Chinese type G gamma+(A gamma delta beta) thal |
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The primer group, the kit and the method for detecting the Chinese type G gamma+(A gamma delta beta) thalassemia deletion variation aim to solve the problems that Gap-PCR and MLPA technologies conventionally adopted in laboratories at present cannot accurately detect fracture sites, the throughput is low, the cost is high, the operation is tedious, the reading length of second-generation high-throughput sequencing is long, and complex structure variation of thalassemia gene clusters cannot be detected. According to the primer group, the kit and the method for detecting the Chinese type G gamma+(A gamma delta beta) thalassemia deletion variation, accurate and rapid detection of Chinese type G gamma+(A gamma delta beta) thal</abstract><oa>free_for_read</oa></addata></record> |
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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE |
| title | Primer group, kit and method for detecting Chinese type G gamma+(A gamma delta beta) thalassemia deletion variation |
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