Polygala tenuifolia medicinal material quality detection method
The invention discloses a polygala tenuifolia medicinal material quality detection method. The method comprises the following steps: step 1, respectively preparing a test sample, a reference substance and a reference medicinal material solution; step 2, constructing HPLC characteristic chromatograms...
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Format: | Patent |
Sprache: | chi ; eng |
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Zusammenfassung: | The invention discloses a polygala tenuifolia medicinal material quality detection method. The method comprises the following steps: step 1, respectively preparing a test sample, a reference substance and a reference medicinal material solution; step 2, constructing HPLC characteristic chromatograms of the polygala tenuifolia medicinal material to be detected, the reference substance and the polygala tenuifolia reference medicinal material under the chromatographic conditions that octadecylsilane chemically bonded silica is used as a filler, acetonitrile is used as a mobile phase A, 0.05% phosphoric acid is used as a mobile phase B, gradient elution is performed, the flow velocity is 0.9-1.1 ml/min, the column temperature is 38-42 DEG C, the detection wavelength is 318-322 nm, and the sample size is 10 microliters; step 3, comparing the HPLC characteristic chromatogram of the polygala tenuifolia medicinal material to be detected with the HPLC characteristic chromatogram of the reference substance and the HPLC |
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