Engineering bacterium of 3-methyl-4-nitrobenzoic acid and preparation method of engineering bacterium
The invention relates to a method for preparing 3-methyl-4-nitrobenzoic acid by using microorganisms and belongs to the technical field of biochemical engineering. The invention provides a recombinant Escherichia coli strain which is E. coli BL21-pRSFDuet-1-L163W, a nucleotide sequence of the recomb...
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| creator | SHAO XIANXIANG MEN JINMING ZHAI TIANLONG XIA JUNGANG CONG RIGANG MIAO HUAMING |
| description | The invention relates to a method for preparing 3-methyl-4-nitrobenzoic acid by using microorganisms and belongs to the technical field of biochemical engineering. The invention provides a recombinant Escherichia coli strain which is E. coli BL21-pRSFDuet-1-L163W, a nucleotide sequence of the recombinant Escherichia coli strain is shown as SEQ ID NO: 3, a corresponding amino acid sequence of the recombinant Escherichia coli strain is shown as SEQ ID NO: 4, and the invention also provides application of the recombinant Escherichia coli strain in preparation of the 3-methyl-4-nitrobenzoic acid. The invention provides a process for preparing and producing the 3-methyl-4-nitrobenzoic acid through a microbiological method. The problem that in the prior art, a catalyst and an oxidizing agent are high in price or serious in pollution or potential safety hazards exist in a production process is solved.
本发明涉及一种利用微生物制备3-甲基-4-硝基苯甲酸的方法，属于生物化工技术领域。本发明提供了一种重组大肠杆菌菌株，为E.coli BL21-pRSFDuet-1-L163W，其核苷酸序列如SEQ ID NO:3所示，其对应的氨基酸 |
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本发明涉及一种利用微生物制备3-甲基-4-硝基苯甲酸的方法，属于生物化工技术领域。本发明提供了一种重组大肠杆菌菌株，为E.coli BL21-pRSFDuet-1-L163W，其核苷酸序列如SEQ ID NO:3所示，其对应的氨基酸</description><language>chi ; eng</language><subject>BEER ; BIOCHEMISTRY ; CHEMISTRY ; COMPOSITIONS THEREOF ; CULTURE MEDIA ; ENZYMOLOGY ; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE ; METALLURGY ; MICROBIOLOGY ; MICROORGANISMS OR ENZYMES ; MUTATION OR GENETIC ENGINEERING ; PROCESSES USING MICROORGANISMS ; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS ; SPIRITS ; VINEGAR ; WINE</subject><creationdate>2022</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20220107&DB=EPODOC&CC=CN&NR=113897322A$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,776,881,25542,76516</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20220107&DB=EPODOC&CC=CN&NR=113897322A$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>SHAO XIANXIANG</creatorcontrib><creatorcontrib>MEN JINMING</creatorcontrib><creatorcontrib>ZHAI TIANLONG</creatorcontrib><creatorcontrib>XIA JUNGANG</creatorcontrib><creatorcontrib>CONG RIGANG</creatorcontrib><creatorcontrib>MIAO HUAMING</creatorcontrib><title>Engineering bacterium of 3-methyl-4-nitrobenzoic acid and preparation method of engineering bacterium</title><description>The invention relates to a method for preparing 3-methyl-4-nitrobenzoic acid by using microorganisms and belongs to the technical field of biochemical engineering. The invention provides a recombinant Escherichia coli strain which is E. coli BL21-pRSFDuet-1-L163W, a nucleotide sequence of the recombinant Escherichia coli strain is shown as SEQ ID NO: 3, a corresponding amino acid sequence of the recombinant Escherichia coli strain is shown as SEQ ID NO: 4, and the invention also provides application of the recombinant Escherichia coli strain in preparation of the 3-methyl-4-nitrobenzoic acid. The invention provides a process for preparing and producing the 3-methyl-4-nitrobenzoic acid through a microbiological method. The problem that in the prior art, a catalyst and an oxidizing agent are high in price or serious in pollution or potential safety hazards exist in a production process is solved.
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本发明涉及一种利用微生物制备3-甲基-4-硝基苯甲酸的方法，属于生物化工技术领域。本发明提供了一种重组大肠杆菌菌株，为E.coli BL21-pRSFDuet-1-L163W，其核苷酸序列如SEQ ID NO:3所示，其对应的氨基酸</abstract><oa>free_for_read</oa></addata></record> |
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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE |
| title | Engineering bacterium of 3-methyl-4-nitrobenzoic acid and preparation method of engineering bacterium |
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