Method for obtaining high-virulence Seneca virus vaccine seed virus and application of high-virulence Seneca virus vaccine seed virus

The invention discloses a method for obtaining a high-virulence Seneca virus vaccine seed virus and application of the high-virulence Seneca virus vaccine seed virus, and belongs to the technical field of veterinary biological products. The method provided by the invention comprises the following steps of selecting diseased tissues with the most serious clinical symptom expression of diseased pigs infected by the Seneca virus original virus, carrying out antigen separation on the diseased tissues, and carrying out cell culture and continuous multiple cloning purification to obtain the high-virulence Seneca virus vaccine seed virus. The method provided by the invention can effectively obtain the Seneca virus vaccine seed virus with high titer and strong immunogenicity, and can be used for producing the Seneca virus vaccine with a particularly ideal immune effect. 本发明公开一种获得高毒力塞内卡病毒疫苗种毒的方法及其应用，属于兽用生物制品技术领域。本发明提供的方法包括选取经塞内卡病毒原始毒感染后的发病猪的临床症状表现最严重的发病组织，并对发病组织进行抗原分离，经细胞培养和连续多次克隆纯化获得高毒力的塞内卡病毒疫苗种毒。本发明方法能够有效获得高滴度且免疫原性强