Primer composition and kit for rapidly detecting variation of SARS-CoV-2 Delta variant
The invention provides a primer composition and a kit for rapidly detecting the variation of an SARS-CoV-2Delta variant strain. The primer composition comprises a primer system aiming at L452R and a primer system aiming at T478K; the primer system for the L452R comprises primers and a probe, wherein...
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| creator | CAO GUOJUN ZHANG QINGYUN CHEN YU XING ZHIFANG ZHONG ZHENQIANG GUAN MING ZHU MIN |
| description | The invention provides a primer composition and a kit for rapidly detecting the variation of an SARS-CoV-2Delta variant strain. The primer composition comprises a primer system aiming at L452R and a primer system aiming at T478K; the primer system for the L452R comprises primers and a probe, wherein the sequences of the primers are SEQ ID NO.2 to SEQ ID NO.6 or the sequences of the primers are SEQ ID NO.8 to SEQ ID NO.12, and a probe wherein the sequence of the probe is SEQ ID NO.7; the primer system for the T478K comprises primers of which the sequences are SEQ ID NO.14 to SEQ ID NO.19, or primers of which the sequences are SEQ ID NO.15, SEQ ID NO.17 and SEQ ID NO.21 to SEQ ID NO.23, or primers of which the sequences are SEQ ID NO.14 to SEQ ID NO.17 and SEQ ID NO.23 to SEQ ID NO.24, or primers of which the sequences are SEQ ID NO.15, SEQ ID NO.17, SEQ ID NO.23 and SEQ ID NO.25 to SEQ ID NO.26, and a probe of which the sequence is SEQ ID NO.20. The primer composition is high in sensitivity and strong in speci |
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The primer composition comprises a primer system aiming at L452R and a primer system aiming at T478K; the primer system for the L452R comprises primers and a probe, wherein the sequences of the primers are SEQ ID NO.2 to SEQ ID NO.6 or the sequences of the primers are SEQ ID NO.8 to SEQ ID NO.12, and a probe wherein the sequence of the probe is SEQ ID NO.7; the primer system for the T478K comprises primers of which the sequences are SEQ ID NO.14 to SEQ ID NO.19, or primers of which the sequences are SEQ ID NO.15, SEQ ID NO.17 and SEQ ID NO.21 to SEQ ID NO.23, or primers of which the sequences are SEQ ID NO.14 to SEQ ID NO.17 and SEQ ID NO.23 to SEQ ID NO.24, or primers of which the sequences are SEQ ID NO.15, SEQ ID NO.17, SEQ ID NO.23 and SEQ ID NO.25 to SEQ ID NO.26, and a probe of which the sequence is SEQ ID NO.20. 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The primer composition comprises a primer system aiming at L452R and a primer system aiming at T478K; the primer system for the L452R comprises primers and a probe, wherein the sequences of the primers are SEQ ID NO.2 to SEQ ID NO.6 or the sequences of the primers are SEQ ID NO.8 to SEQ ID NO.12, and a probe wherein the sequence of the probe is SEQ ID NO.7; the primer system for the T478K comprises primers of which the sequences are SEQ ID NO.14 to SEQ ID NO.19, or primers of which the sequences are SEQ ID NO.15, SEQ ID NO.17 and SEQ ID NO.21 to SEQ ID NO.23, or primers of which the sequences are SEQ ID NO.14 to SEQ ID NO.17 and SEQ ID NO.23 to SEQ ID NO.24, or primers of which the sequences are SEQ ID NO.15, SEQ ID NO.17, SEQ ID NO.23 and SEQ ID NO.25 to SEQ ID NO.26, and a probe of which the sequence is SEQ ID NO.20. 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The primer composition comprises a primer system aiming at L452R and a primer system aiming at T478K; the primer system for the L452R comprises primers and a probe, wherein the sequences of the primers are SEQ ID NO.2 to SEQ ID NO.6 or the sequences of the primers are SEQ ID NO.8 to SEQ ID NO.12, and a probe wherein the sequence of the probe is SEQ ID NO.7; the primer system for the T478K comprises primers of which the sequences are SEQ ID NO.14 to SEQ ID NO.19, or primers of which the sequences are SEQ ID NO.15, SEQ ID NO.17 and SEQ ID NO.21 to SEQ ID NO.23, or primers of which the sequences are SEQ ID NO.14 to SEQ ID NO.17 and SEQ ID NO.23 to SEQ ID NO.24, or primers of which the sequences are SEQ ID NO.15, SEQ ID NO.17, SEQ ID NO.23 and SEQ ID NO.25 to SEQ ID NO.26, and a probe of which the sequence is SEQ ID NO.20. The primer composition is high in sensitivity and strong in speci</abstract><oa>free_for_read</oa></addata></record> |
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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE |
| title | Primer composition and kit for rapidly detecting variation of SARS-CoV-2 Delta variant |
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