Pig circRNA sequence, application thereof and cyclization identification method

The invention is based on the most common intestinal inflammatory disease, namely piglet bacterial diarrhea (PDW), wherein the F18 Escherichia coli (E. coli F18) is a main pathogen causing weaned piglet bacterial diarrhea, and regulation mechanisms of local variety pigs to the resistance of the E. coli F18 are to be systematically clarified. In the early stage, a key candidate RNA-circRNA-27388 for escherichia coli F18 infection regulation is screened out through omics sequencing, as a circRNA-27388 body gene is FUT8, the circRNA-27388 body gene is named as circ-FUT8, on the basis, the circ-FUT8 sequence is subjected to cyclization identification, and a foundation is laid for follow-up verification of circRNA functions and the regulation mechanisms. 本发明基于最常见的一类肠道炎症性疾病--仔猪细菌性腹泻（PDW），其中F18大肠杆菌（E.coli F18）是引起断奶仔猪细菌性腹泻的主要病原，而地方品种猪对其抗性的调控机制有待系统阐明。前期通过组学测序筛选出1个大肠杆菌F18感染调控的关键候选RNA-circRNA_27388，由于circRNA_27388本体基因为FUT8，因此将其命名为circ_FUT8，在此基础上，本研究对circ_FUT8序列进行成环鉴定，为后续验证circRNA功能及调控机制奠定基础。