Plebopus portentosus sporocarp polysaccharide and extraction, separation and purification method thereof

Plebopus portentosus sporocarp polysaccharide and extraction, separation and purification method thereof

The invention discloses a phlebopus portentosus sporocarp polysaccharide and an extraction, separation and purification method thereof. The phlebopus portentosus sporocarp polysaccharide is composed of mannose Man, glucose Glc, galactose Gal and L-fucose Fuc. The extraction, separation and purificat...

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Hauptverfasser: LI HUANCHANG, XIE HUIJING, ZHANG YUZHUO, ZENG NIANKAI, HAN YUNXIAO, ZHANG XU, WANG YONG, XU CHANG, TIAN RUN
Format: Patent
Sprache:chi ; eng
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CHEMISTRY
COMPOSITIONS BASED THEREON
DERIVATIVES THEREOF
METALLURGY
ORGANIC MACROMOLECULAR COMPOUNDS
POLYSACCHARIDES
THEIR PREPARATION OR CHEMICAL WORKING-UP
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creator LI HUANCHANG
XIE HUIJING
ZHANG YUZHUO
ZENG NIANKAI
HAN YUNXIAO
ZHANG XU
WANG YONG
XU CHANG
TIAN RUN
description The invention discloses a phlebopus portentosus sporocarp polysaccharide and an extraction, separation and purification method thereof. The phlebopus portentosus sporocarp polysaccharide is composed of mannose Man, glucose Glc, galactose Gal and L-fucose Fuc. The extraction, separation and purification method comprises the following steps: drying phlebopus portentosus sporocarp at low temperature, and then smashing the phlebopus portentosus sporocarp; preparing crude polysaccharide by adopting a water extraction and alcohol precipitation method, removing protein by adopting a Sevage reagent, removing pigment by adopting SP825 macroporous adsorption resin, separating and purifying by adopting DEAE-52 cellulose column chromatography, dialyzing by adopting a dialysis bag to remove inorganic salt and small molecular impurities, and further separating and purifying by using propylene sephadex S-400HR to prepare refined polysaccharide. The polysaccharide is prepared from the sporocarp of the phlebopus portentosus,
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subjects CHEMISTRY
COMPOSITIONS BASED THEREON
DERIVATIVES THEREOF
METALLURGY
ORGANIC MACROMOLECULAR COMPOUNDS
POLYSACCHARIDES
THEIR PREPARATION OR CHEMICAL WORKING-UP
title Plebopus portentosus sporocarp polysaccharide and extraction, separation and purification method thereof
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