Method for capturing blood circulating tumor cells
The invention provides a method for capturing blood circulating tumor cells. The method comprises the following steps of S1, extracting a set amount of peripheral blood by using an ACD anticoagulation tube according to requirements, and upside down and uniformly mixing the peripheral blood, adding a...
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| creator | BAO WEISHENG WANG CHUNLEI BAO YUEYANG |
| description | The invention provides a method for capturing blood circulating tumor cells. The method comprises the following steps of S1, extracting a set amount of peripheral blood by using an ACD anticoagulation tube according to requirements, and upside down and uniformly mixing the peripheral blood, adding a platelet inhibitor, and storing or transporting at a low temperature of 4-8 DEG C; S2, taking out the blood from the low-temperature condition to be placed at the room temperature, namely 20-25 DEG C to be balanced to the set time, adding EDTA, performing uniform mixing out, and performing balancing at the room temperature; and S3, performing blood filtration on the balanced blood, and capturing tumor cells. According to the method for capturing the blood circulating tumor cells, storage, stabilization and transportation of whole blood containing rare cells such as CTC are facilitated, the method is matched with a CTC chip separation technology based on the form and size of the tumor cells, and the CTC capturing p |
| format | Patent |
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The method comprises the following steps of S1, extracting a set amount of peripheral blood by using an ACD anticoagulation tube according to requirements, and upside down and uniformly mixing the peripheral blood, adding a platelet inhibitor, and storing or transporting at a low temperature of 4-8 DEG C; S2, taking out the blood from the low-temperature condition to be placed at the room temperature, namely 20-25 DEG C to be balanced to the set time, adding EDTA, performing uniform mixing out, and performing balancing at the room temperature; and S3, performing blood filtration on the balanced blood, and capturing tumor cells. 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The method comprises the following steps of S1, extracting a set amount of peripheral blood by using an ACD anticoagulation tube according to requirements, and upside down and uniformly mixing the peripheral blood, adding a platelet inhibitor, and storing or transporting at a low temperature of 4-8 DEG C; S2, taking out the blood from the low-temperature condition to be placed at the room temperature, namely 20-25 DEG C to be balanced to the set time, adding EDTA, performing uniform mixing out, and performing balancing at the room temperature; and S3, performing blood filtration on the balanced blood, and capturing tumor cells. According to the method for capturing the blood circulating tumor cells, storage, stabilization and transportation of whole blood containing rare cells such as CTC are facilitated, the method is matched with a CTC chip separation technology based on the form and size of the tumor cells, and the CTC capturing p</description><subject>AGRICULTURE</subject><subject>ANIMAL HUSBANDRY</subject><subject>BEER</subject><subject>BIOCHEMISTRY</subject><subject>BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES, AS HERBICIDES</subject><subject>CHEMISTRY</subject><subject>COMPOSITIONS THEREOF</subject><subject>CULTURE MEDIA</subject><subject>ENZYMOLOGY</subject><subject>FISHING</subject><subject>FORESTRY</subject><subject>HUMAN NECESSITIES</subject><subject>HUNTING</subject><subject>METALLURGY</subject><subject>MICROBIOLOGY</subject><subject>MICROORGANISMS OR ENZYMES</subject><subject>MUTATION OR GENETIC ENGINEERING</subject><subject>PEST REPELLANTS OR ATTRACTANTS</subject><subject>PLANT GROWTH REGULATORS</subject><subject>PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTSTHEREOF</subject><subject>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</subject><subject>SPIRITS</subject><subject>TRAPPING</subject><subject>VINEGAR</subject><subject>WINE</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>2021</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNrjZDDyTS3JyE9RSMsvUkhOLCgpLcrMS1dIyskHiiVnFiWX5iSWgERKSnNBKlJzcop5GFjTEnOKU3mhNDeDoptriLOHbmpBfnxqcUFicmpeakm8s5-hobGZsbmZsaWjMTFqANyALEs</recordid><startdate>20211112</startdate><enddate>20211112</enddate><creator>BAO WEISHENG</creator><creator>WANG CHUNLEI</creator><creator>BAO YUEYANG</creator><scope>EVB</scope></search><sort><creationdate>20211112</creationdate><title>Method for capturing blood circulating tumor cells</title><author>BAO WEISHENG ; WANG CHUNLEI ; BAO YUEYANG</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_CN113637639A3</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>chi ; eng</language><creationdate>2021</creationdate><topic>AGRICULTURE</topic><topic>ANIMAL HUSBANDRY</topic><topic>BEER</topic><topic>BIOCHEMISTRY</topic><topic>BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES, AS HERBICIDES</topic><topic>CHEMISTRY</topic><topic>COMPOSITIONS THEREOF</topic><topic>CULTURE MEDIA</topic><topic>ENZYMOLOGY</topic><topic>FISHING</topic><topic>FORESTRY</topic><topic>HUMAN NECESSITIES</topic><topic>HUNTING</topic><topic>METALLURGY</topic><topic>MICROBIOLOGY</topic><topic>MICROORGANISMS OR ENZYMES</topic><topic>MUTATION OR GENETIC ENGINEERING</topic><topic>PEST REPELLANTS OR ATTRACTANTS</topic><topic>PLANT GROWTH REGULATORS</topic><topic>PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTSTHEREOF</topic><topic>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</topic><topic>SPIRITS</topic><topic>TRAPPING</topic><topic>VINEGAR</topic><topic>WINE</topic><toplevel>online_resources</toplevel><creatorcontrib>BAO WEISHENG</creatorcontrib><creatorcontrib>WANG CHUNLEI</creatorcontrib><creatorcontrib>BAO YUEYANG</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>BAO WEISHENG</au><au>WANG CHUNLEI</au><au>BAO YUEYANG</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>Method for capturing blood circulating tumor cells</title><date>2021-11-12</date><risdate>2021</risdate><abstract>The invention provides a method for capturing blood circulating tumor cells. The method comprises the following steps of S1, extracting a set amount of peripheral blood by using an ACD anticoagulation tube according to requirements, and upside down and uniformly mixing the peripheral blood, adding a platelet inhibitor, and storing or transporting at a low temperature of 4-8 DEG C; S2, taking out the blood from the low-temperature condition to be placed at the room temperature, namely 20-25 DEG C to be balanced to the set time, adding EDTA, performing uniform mixing out, and performing balancing at the room temperature; and S3, performing blood filtration on the balanced blood, and capturing tumor cells. According to the method for capturing the blood circulating tumor cells, storage, stabilization and transportation of whole blood containing rare cells such as CTC are facilitated, the method is matched with a CTC chip separation technology based on the form and size of the tumor cells, and the CTC capturing p</abstract><oa>free_for_read</oa></addata></record> |
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| language | chi ; eng |
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| subjects | AGRICULTURE ANIMAL HUSBANDRY BEER BIOCHEMISTRY BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES, AS HERBICIDES CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FISHING FORESTRY HUMAN NECESSITIES HUNTING METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PEST REPELLANTS OR ATTRACTANTS PLANT GROWTH REGULATORS PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTSTHEREOF PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS TRAPPING VINEGAR WINE |
| title | Method for capturing blood circulating tumor cells |
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