Strawberry genetic transformation system construction method

The invention discloses a genetic transformation system of a new strawberry variety 'Shenqi'. An agrobacterium tumefaciens-mediated 'Shenqi' leaf and petiole genetic transformation system is established through a leaf and petiole in-vitro regeneration method, and the method comprises the following steps: preparing a sterile leaf; screening the optimal exogenous hormone concentration to induce the leaves and petioles of the strawberries' Shenqi 'to regenerate adventitious buds; analyzing the sensitivity of 'Shenqi' leaf regeneration on antibiotics; activating and culturing agrobacterium tumefaciens; inducing and culturing adventitious buds of the leaves and the petioles; soaking leaves with agrobacterium and performing co-culturing; screening positive strains with antibiotics; and detecting a transformed strain and the like. According to the method disclosed by the invention, adventitious buds are induced by utilizing strawberry leaves and petioles, the leaves are impregnated by agrobacterium, and transformed