Fusion protein for cytosine base editing and application thereof

The invention relates to the field of molecular biology, in particular to a fusion protein for cytosine base editing and application of thereof. The fusion protein comprises a) a Cas9 enzyme domain; b) an unstable domain (DD); c) a cytosine deaminase domain (CDA) and d) a uracil glycosidase inhibitor (UGI) domain. According to the invention, high-efficiency, high-precision and controllable base editing can be realized in genomes of various eukaryotes. And high-precision and high-efficiency C-T base editing can be carried out in genomes of various eukaryotes under the condition that deletion and insertion are not introduced. 本发明涉及分子生物学领域，具体而言，涉及一种用于胞嘧啶碱基编辑的融合蛋白及其应用。该融合蛋白包含a)Cas9酶域；b)不稳定结构域(DD)；c)胞嘧啶脱氨酶域(CDA)以及d)尿嘧啶糖苷酶抑制剂(UGI)域。其能够在各种真核生物的基因组中实现高效率、高精度、可调控的碱基编辑。并能够在不引入缺失和插入的情况下，在各种真核生物的基因组中实施高精度高功效的C-T碱基编辑。