Brain microvascular endothelial cell extraction and purification method
The invention discloses a brain microvascular endothelial cell extraction and purification method, and relates to the technical field of cell purification. The extraction and purification method comprises the following steps: S1, coarsely extracting brain microvascular endothelial cells in brain tis...
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| creator | LU HAIKE HUANG ZHIXIN LIU XINTONG |
| description | The invention discloses a brain microvascular endothelial cell extraction and purification method, and relates to the technical field of cell purification. The extraction and purification method comprises the following steps: S1, coarsely extracting brain microvascular endothelial cells in brain tissues of a mouse; S2, carrying out primary CD31 immunomagnetic bead sorting on a brain microvascular endothelial cell mixture treated in the step S1 for purification; and S3, carrying out secondary CD31 immunomagnetic bead sorting on brain microvascular endothelial cells treated in the step S2 for purification so as to obtain the purified brain microvascular endothelial cells. According to the method, a mode of carrying out CD31 magnetic bead sorting for twice is adopted, damage to the activity of the brain microvascular endothelial cells is reduced, the separation and purification efficiency is high, the operation steps are relatively simple, and the brain microvascular endothelial cells with high purity, high yiel |
| format | Patent |
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The extraction and purification method comprises the following steps: S1, coarsely extracting brain microvascular endothelial cells in brain tissues of a mouse; S2, carrying out primary CD31 immunomagnetic bead sorting on a brain microvascular endothelial cell mixture treated in the step S1 for purification; and S3, carrying out secondary CD31 immunomagnetic bead sorting on brain microvascular endothelial cells treated in the step S2 for purification so as to obtain the purified brain microvascular endothelial cells. 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The extraction and purification method comprises the following steps: S1, coarsely extracting brain microvascular endothelial cells in brain tissues of a mouse; S2, carrying out primary CD31 immunomagnetic bead sorting on a brain microvascular endothelial cell mixture treated in the step S1 for purification; and S3, carrying out secondary CD31 immunomagnetic bead sorting on brain microvascular endothelial cells treated in the step S2 for purification so as to obtain the purified brain microvascular endothelial cells. According to the method, a mode of carrying out CD31 magnetic bead sorting for twice is adopted, damage to the activity of the brain microvascular endothelial cells is reduced, the separation and purification efficiency is high, the operation steps are relatively simple, and the brain microvascular endothelial cells with high purity, high yiel</abstract><oa>free_for_read</oa></addata></record> |
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| title | Brain microvascular endothelial cell extraction and purification method |
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