Preparation method of penicillium griseofulvum for efficiently degrading cellulose

The invention discloses a screening method of penicillium griseofulvum for efficiently degrading cellulose. The screening method comprises the following steps of: activating and enriching obtained flora on a universal culture medium PDA (Potato dextrose agar) and purifying the flora; taking a filter...

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Hauptverfasser: GAO XING'AI, QI CHUNYAN, NA WEI, LI HAIYAN, HOU LIGANG, GUAN FACHUN, CAI JINLING, WANG LIXIA, OU WEI, ZHAI XIANGYING, ZHANG YONGFENG, XIE JIAO, WANG CHAO, ZHAO XINYING, MA WEI
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creator GAO XING'AI
QI CHUNYAN
NA WEI
LI HAIYAN
HOU LIGANG
GUAN FACHUN
CAI JINLING
WANG LIXIA
OU WEI
ZHAI XIANGYING
ZHANG YONGFENG
XIE JIAO
WANG CHAO
ZHAO XINYING
MA WEI
description The invention discloses a screening method of penicillium griseofulvum for efficiently degrading cellulose. The screening method comprises the following steps of: activating and enriching obtained flora on a universal culture medium PDA (Potato dextrose agar) and purifying the flora; taking a filter paper strip as a cellulose degradation indicator; when the indicator in the culture medium is disintegrated, obtaining efficient cellulose degradation bacteria; and finally, carrying out enzyme activity determination. By adopting the method disclosed by the invention, the penicillium griseofulvum with stable performance can be effectively screened. The invention further discloses an application method of the penicillium griseofulvum for efficiently degrading the cellulose; and the application method can be used for degrading rice straws and can form mycelium pellets in the culture medium. 本发现公开了一种纤维素降解灰黄青霉菌筛选方法。将得到的菌群,在通用培养基PDA上活化富集,对菌群进行纯化。以滤纸条为纤维素降解指示物,当培养基内指示物崩解,即可得到高效的纤维素降解菌,最后进行酶活测定。通过上述方法,能够有效地筛选到性能稳定的纤维素降解菌
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The screening method comprises the following steps of: activating and enriching obtained flora on a universal culture medium PDA (Potato dextrose agar) and purifying the flora; taking a filter paper strip as a cellulose degradation indicator; when the indicator in the culture medium is disintegrated, obtaining efficient cellulose degradation bacteria; and finally, carrying out enzyme activity determination. By adopting the method disclosed by the invention, the penicillium griseofulvum with stable performance can be effectively screened. The invention further discloses an application method of the penicillium griseofulvum for efficiently degrading the cellulose; and the application method can be used for degrading rice straws and can form mycelium pellets in the culture medium. 本发现公开了一种纤维素降解灰黄青霉菌筛选方法。将得到的菌群,在通用培养基PDA上活化富集,对菌群进行纯化。以滤纸条为纤维素降解指示物,当培养基内指示物崩解,即可得到高效的纤维素降解菌,最后进行酶活测定。通过上述方法,能够有效地筛选到性能稳定的纤维素降解菌</abstract><oa>free_for_read</oa></addata></record>
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subjects BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROCESSES USING MICROORGANISMS
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
title Preparation method of penicillium griseofulvum for efficiently degrading cellulose
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