Method for efficient 3D culture of adipose-derived stem cells

The invention relates to the technical field of adipose-derived stem cell culture, in particular to a method for the efficient 3D culture of adipose-derived stem cells, which comprises the following steps: (1) obtaining adipose-derived stem cells; (2) after cell counting, re-suspending the adipose-derived stem cells in hydrogel, and carrying out 3D suspension culture; and (3) after the cells grow to the density of 80-90%, carrying out 1: 2 passage, and continuing to culture in the hydrogel, the efficient 3D adipose-derived stem cell culture method can maintain the cell morphology, delay the aging and multidirectional differentiation of the stem cells, maintain the stemness and the cell biological activity, provide a reliable culture mode for the in-vitro experimental research of the stem cells. The possibility is provided for in-vitro high simulation of an in-vivo experiment. 本发明涉及脂肪干细胞培养技术领域，具体是一种高效3D培养脂肪干细胞的方法，包括以下步骤：（1）脂肪干细胞的获得；（2）细胞计数后，将脂肪干细胞重悬于水凝胶中，进行3D悬浮培养；（3）细胞生长到80-90%密度后进行1：2传代继续于水凝胶中培养，本发明高效3D培养脂肪干细