Recombinant SGLT2 protein and application thereof
The invention provides a recombinant SGLT2 protein and an application thereof. The recombinant SGLT2 protein comprises an amino acid sequence as shown in SEQ ID No. 1. The invention also provides a nucleic acid molecule, a recombinant plasmid, a recombinant virus, a recombinant cell and a preparatio...
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| creator | ZHANG JIN LI JIAN ZHANG YUTING NAN WEIWEI WAN SHUANGYAN |
| description | The invention provides a recombinant SGLT2 protein and an application thereof. The recombinant SGLT2 protein comprises an amino acid sequence as shown in SEQ ID No. 1. The invention also provides a nucleic acid molecule, a recombinant plasmid, a recombinant virus, a recombinant cell and a preparation method of the recombinant SGLT2 protein. The recombinant SGLT2 protein has good folding and post-translational processing levels, is very close to protein in a natural state, and is good in biological activity, high in stability and good in water solubility; the preparation method of the recombinant SGLT2 protein is mature in technology, high in success rate, easy to master, high in yield of the recombinant protein, good in purification effect and wide in application prospect.
本发明提供了一种重组SGLT2蛋白及其应用，所述重组SGLT2蛋白包括SEQ ID No.1所示的氨基酸序列。本发明还提供了一种核酸分子、一种重组质粒、一种重组病毒、一种重组细胞以及所述重组SGLT2蛋白的制备方法。所述重组SGLT2蛋白具有良好的折叠以及翻译后加工的水平，与天然状态下的蛋白十分接近，生物活性好，稳定性高，水溶性好；所述重组SGLT2蛋白的制备方法技术成熟，成功率高，容易掌握，重组蛋白的产量高，纯化效果好，应用前景广阔。 |
| format | Patent |
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本发明提供了一种重组SGLT2蛋白及其应用，所述重组SGLT2蛋白包括SEQ ID No.1所示的氨基酸序列。本发明还提供了一种核酸分子、一种重组质粒、一种重组病毒、一种重组细胞以及所述重组SGLT2蛋白的制备方法。所述重组SGLT2蛋白具有良好的折叠以及翻译后加工的水平，与天然状态下的蛋白十分接近，生物活性好，稳定性高，水溶性好；所述重组SGLT2蛋白的制备方法技术成熟，成功率高，容易掌握，重组蛋白的产量高，纯化效果好，应用前景广阔。</description><language>chi ; eng</language><subject>BEER ; BIOCHEMISTRY ; CHEMISTRY ; COMPOSITIONS THEREOF ; CULTURE MEDIA ; ENZYMOLOGY ; METALLURGY ; MICROBIOLOGY ; MICROORGANISMS OR ENZYMES ; MUTATION OR GENETIC ENGINEERING ; ORGANIC CHEMISTRY ; PEPTIDES ; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS ; SPIRITS ; VINEGAR ; WINE</subject><creationdate>2021</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20211001&DB=EPODOC&CC=CN&NR=113461796A$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,776,881,25542,76290</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20211001&DB=EPODOC&CC=CN&NR=113461796A$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>ZHANG JIN</creatorcontrib><creatorcontrib>LI JIAN</creatorcontrib><creatorcontrib>ZHANG YUTING</creatorcontrib><creatorcontrib>NAN WEIWEI</creatorcontrib><creatorcontrib>WAN SHUANGYAN</creatorcontrib><title>Recombinant SGLT2 protein and application thereof</title><description>The invention provides a recombinant SGLT2 protein and an application thereof. The recombinant SGLT2 protein comprises an amino acid sequence as shown in SEQ ID No. 1. The invention also provides a nucleic acid molecule, a recombinant plasmid, a recombinant virus, a recombinant cell and a preparation method of the recombinant SGLT2 protein. The recombinant SGLT2 protein has good folding and post-translational processing levels, is very close to protein in a natural state, and is good in biological activity, high in stability and good in water solubility; the preparation method of the recombinant SGLT2 protein is mature in technology, high in success rate, easy to master, high in yield of the recombinant protein, good in purification effect and wide in application prospect.
本发明提供了一种重组SGLT2蛋白及其应用，所述重组SGLT2蛋白包括SEQ ID No.1所示的氨基酸序列。本发明还提供了一种核酸分子、一种重组质粒、一种重组病毒、一种重组细胞以及所述重组SGLT2蛋白的制备方法。所述重组SGLT2蛋白具有良好的折叠以及翻译后加工的水平，与天然状态下的蛋白十分接近，生物活性好，稳定性高，水溶性好；所述重组SGLT2蛋白的制备方法技术成熟，成功率高，容易掌握，重组蛋白的产量高，纯化效果好，应用前景广阔。</description><subject>BEER</subject><subject>BIOCHEMISTRY</subject><subject>CHEMISTRY</subject><subject>COMPOSITIONS THEREOF</subject><subject>CULTURE MEDIA</subject><subject>ENZYMOLOGY</subject><subject>METALLURGY</subject><subject>MICROBIOLOGY</subject><subject>MICROORGANISMS OR ENZYMES</subject><subject>MUTATION OR GENETIC ENGINEERING</subject><subject>ORGANIC CHEMISTRY</subject><subject>PEPTIDES</subject><subject>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</subject><subject>SPIRITS</subject><subject>VINEGAR</subject><subject>WINE</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>2021</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNrjZDAMSk3Oz03KzEvMK1EIdvcJMVIoKMovSc3MU0jMS1FILCjIyUxOLMnMz1MoyUgtSs1P42FgTUvMKU7lhdLcDIpuriHOHrqpBfnxqcUFicmpeakl8c5-hobGJmaG5pZmjsbEqAEAZEIrVw</recordid><startdate>20211001</startdate><enddate>20211001</enddate><creator>ZHANG JIN</creator><creator>LI JIAN</creator><creator>ZHANG YUTING</creator><creator>NAN WEIWEI</creator><creator>WAN SHUANGYAN</creator><scope>EVB</scope></search><sort><creationdate>20211001</creationdate><title>Recombinant SGLT2 protein and application thereof</title><author>ZHANG JIN ; LI JIAN ; ZHANG YUTING ; NAN WEIWEI ; WAN SHUANGYAN</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_CN113461796A3</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>chi ; eng</language><creationdate>2021</creationdate><topic>BEER</topic><topic>BIOCHEMISTRY</topic><topic>CHEMISTRY</topic><topic>COMPOSITIONS THEREOF</topic><topic>CULTURE MEDIA</topic><topic>ENZYMOLOGY</topic><topic>METALLURGY</topic><topic>MICROBIOLOGY</topic><topic>MICROORGANISMS OR ENZYMES</topic><topic>MUTATION OR GENETIC ENGINEERING</topic><topic>ORGANIC CHEMISTRY</topic><topic>PEPTIDES</topic><topic>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</topic><topic>SPIRITS</topic><topic>VINEGAR</topic><topic>WINE</topic><toplevel>online_resources</toplevel><creatorcontrib>ZHANG JIN</creatorcontrib><creatorcontrib>LI JIAN</creatorcontrib><creatorcontrib>ZHANG YUTING</creatorcontrib><creatorcontrib>NAN WEIWEI</creatorcontrib><creatorcontrib>WAN SHUANGYAN</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>ZHANG JIN</au><au>LI JIAN</au><au>ZHANG YUTING</au><au>NAN WEIWEI</au><au>WAN SHUANGYAN</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>Recombinant SGLT2 protein and application thereof</title><date>2021-10-01</date><risdate>2021</risdate><abstract>The invention provides a recombinant SGLT2 protein and an application thereof. The recombinant SGLT2 protein comprises an amino acid sequence as shown in SEQ ID No. 1. The invention also provides a nucleic acid molecule, a recombinant plasmid, a recombinant virus, a recombinant cell and a preparation method of the recombinant SGLT2 protein. The recombinant SGLT2 protein has good folding and post-translational processing levels, is very close to protein in a natural state, and is good in biological activity, high in stability and good in water solubility; the preparation method of the recombinant SGLT2 protein is mature in technology, high in success rate, easy to master, high in yield of the recombinant protein, good in purification effect and wide in application prospect.
本发明提供了一种重组SGLT2蛋白及其应用，所述重组SGLT2蛋白包括SEQ ID No.1所示的氨基酸序列。本发明还提供了一种核酸分子、一种重组质粒、一种重组病毒、一种重组细胞以及所述重组SGLT2蛋白的制备方法。所述重组SGLT2蛋白具有良好的折叠以及翻译后加工的水平，与天然状态下的蛋白十分接近，生物活性好，稳定性高，水溶性好；所述重组SGLT2蛋白的制备方法技术成熟，成功率高，容易掌握，重组蛋白的产量高，纯化效果好，应用前景广阔。</abstract><oa>free_for_read</oa></addata></record> |
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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING ORGANIC CHEMISTRY PEPTIDES PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE |
| title | Recombinant SGLT2 protein and application thereof |
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