Immunochromatography reagent strip for detecting canine circovirus antigen and preparation method thereof

The invention discloses an immunochromatography reagent strip for detecting a canine circovirus antigen and a preparation method thereof, and belongs to the technical field of in-vitro immunodiagnosis detection tools, the immunochromatography reagent strip comprises a PVC bottom plate, and is charac...

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Bibliographische Detailangaben
Hauptverfasser: NING BO, GUO GUILING, LIANG HUANKUN, ZAN JIE, CHEN CUICUI, LAI HONGRUI, LI LAIQING, ZHONG SHUHAI
Format: Patent
Sprache:chi ; eng
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Beschreibung
Zusammenfassung:The invention discloses an immunochromatography reagent strip for detecting a canine circovirus antigen and a preparation method thereof, and belongs to the technical field of in-vitro immunodiagnosis detection tools, the immunochromatography reagent strip comprises a PVC bottom plate, and is characterized by further comprising a water absorption pad, a nitrocellulose membrane, a marking pad and a sample pad which are sequentially pasted on the PVC bottom plate; the marking pad is sprayed with a CanineCV antibody-colloidal gold compound, and the nitrocellulose membrane is coated with a quality control line C and a detection line T; the quality control line C is coated with a CanineCV recombinant antigen; and the detection line T is coated with a CanineCV antibody. The method comprises the following steps: (1) preparing a colloidal gold solution; (2) preparing a marking pad; (3) coating a quality control line C and a detection line T; (4) pretreating the sample pad; (5) cutting and assembling; and obtaining a