Reporting system for screening VFM quorum sensing signal quenching bacteria and inhibitors and construction method thereof

The invention discloses a reporting system for screening VFM quorum sensing signal quenching bacteria and inhibitors and a construction method thereof. According to the reporting system VR2, the corn Dick's bacteria MS2 are used as recipient bacteria, and reporter plasmids are transferred into the recipient bacteria to obtain the reporting system VR2; the reporter plasmid is obtained by taking a pPROBE-NT plasmid as a framework and inserting an optimized vfmE promoter in front of a GFP gene. According to the present invention, the green fluorescent protein is adopted as the reporter gene, has high detection sensitivity, can be used for detecting the VFM signal intensity on the premise of unknown VFM signal structure, and provides the new idea and the new method for screening the population quenching biocontrol bacteria and the signal inhibitor of the bacterial soft rot caused by Dickeya, and the development of the new green safety prevention and control strategy. 本发明公开了用于筛选VFM群体感应信号淬灭菌和抑制剂的报告系统及其构建方法。本发明的报告系统