ATTACHING NUCLEOTIDES TO POLYNUCLEOTIDE WITH POLYMERASE

Provided is a method including hybridizing a polynucleotide to a template, contacting a first template nucleotide 5-prime adjacent to a 5-prime-most nucleotide of the plurality of nucleotides that are complementary to the polynucleotide with a polymerase and a charge-tagged nucleotide, wherein the c...

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Bibliographische Detailangaben
Hauptverfasser:	ZHAO YANNAN, VINCENT LUDOVIC, PEISAJOVICH SERGIO, ZHANG YINNA, YANG XIANGYUAN, PUGLIESE KAITLIN, MANDELL JOHN, PRABHU ASHOK, GARCIA ERIN, GRAVINA SILVIA, STUTCHMAN JONATHON
Format:	Patent
Sprache:	chi ; eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	Provided is a method including hybridizing a polynucleotide to a template, contacting a first template nucleotide 5-prime adjacent to a 5-prime-most nucleotide of the plurality of nucleotides that are complementary to the polynucleotide with a polymerase and a charge-tagged nucleotide, wherein the charge-tagged nucleotide is complementary to the first template nucleotide and includes a charge tag attached to a 5-prime polyphosphate of the charge-tagged nucleotide. 本发明提供了一种方法，该方法包括使多核苷酸与模板杂交，以及使与该多核苷酸互补的多个核苷酸中的5'最末端核苷酸相邻的第一模板核苷酸5'端与聚合酶和带电荷标签的核苷酸接触，其中该带电荷标签的核苷酸与该第一模板核苷酸互补，并且包括连接到该带电荷标签的核苷酸的5'端多磷酸的电荷标签。