Saccharomyces cerevisiae large-fragment gene editing method

The invention discloses a saccharomyces cerevisiae large-fragment gene editing method. The editing method comprises the following steps: (1) designing a GIM gene expression cassette, specifically, amplifying an I-SceI gene expression cassette controlled by a galactose promoter from a plasmid pGSHU,...

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Hauptverfasser:	ZHOU SA, LI SHIQI, MA WENJIAN, ZHANG TENGYUE, CHEN TECHANG, MU JIAKANG, DING CHUNYANG
Format:	Patent
Sprache:	chi ; eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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