Digital PCR reaction system for detecting BRAF V600E site and application of digital PCR reaction system

The invention provides a digital PCR reaction system for detecting a BRAF V600E site and application of the digital PCR reaction system. The reaction system comprises a BRAF V600E site specific amplification primer, a wild type probe and a mutant type probe, and the sensitivity and the stability of the whole reaction system are greatly improved by optimizing the concentration ratio of the primer and the probes in the PCR amplification system. The reaction system is suitable for detecting various sample types such as tumor tissues, peripheral blood, plasma and the like, and particularly has high detection sensitivity and accurate and reliable results in trace samples such as free nucleic acid and the like, and has relatively high application value. 本发明提供了一种检测BRAF V600E位点的数字PCR反应体系及其应用。所述反应体系包含BRAF V600E位点的特异性扩增引物和野生型、突变型探针，通过优化PCR扩增体系中引物探针的浓度比例，使得整个反应体系的灵敏度和稳定性大幅度提高。本发明适用于肿瘤组织、外周血、血浆等多种样本类型的检测，尤其在游离核酸等等微量样本中检测灵敏度高，结果准确可靠，具有较高的应用价值。