Method for improving fermentation yield of cordycepin

The invention provides a method for increasing the fermentation yield of cordycepin, and belongs to the technical field of biological engineering. Saccharomyces cerevisiae engineering bacteria capable of producing cordycepin are used as production strains, fermentation conditions are optimized, 0.2%-0.6% (v/v) of a surfactant is added in the middle stage of batch fermentation logarithmic growth, the permeability of yeast cell membranes is changed, and the fermentation yield of a product cordycepin with cytotoxicity is increased. According to the method, the fermentation yield of cordycepin is remarkably increased, the fermentation time is shortened, the technical scheme is simple and environment-friendly, and the genetic stability of the strain is high. The method has important application significance for industrial production of cordycepin. 本发明提供了一种提高虫草素发酵产量的方法，属于生物工程技术领域。本发明是以可产虫草素的酿酒酵母工程菌为生产菌株，优化其发酵条件，在分批发酵对数生长中期添加0.2％-0.6％(v/v)表面活性剂，改变酵母细胞膜通透性，增加具有细胞毒性的产物虫草素的发酵产量。本发明显著提高了虫草素的发酵产量，缩短了发酵时间，技术方案简单、环境友好、菌种遗传