Preparation and application of antibody for bispecific recognition of albendazole and carbendazim
The invention discloses preparation and application of an antibody for bispecific recognition of albendazole and carbendazim. Hapten is coupled to carrier protein to prepare two artificial antigens, the two artificial antigens are respectively used for animal immunization and used as coating antigen...
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| creator | XIAO ZEMIAO CHEN DIAN SHEN YUDONG LIANG ZHIWEN LUO LIN CHEN ZIJIAN SUN YUANMING XU ZHENLIN |
| description | The invention discloses preparation and application of an antibody for bispecific recognition of albendazole and carbendazim. Hapten is coupled to carrier protein to prepare two artificial antigens, the two artificial antigens are respectively used for animal immunization and used as coating antigen, hybridoma cells capable of secreting albendazole and/or carbendazim antibodies are prepared through cell fusion, and further a detection method based on indirect competitive enzyme-linked immunosorbent assay is established by using the coating antigen. The detection method for detecting albendazole and/or carbendazim has the characteristics of strong specificity, high sensitivity, rapidness, simplicity, convenience and the like, wherein the IC50 value of albendazole is 0.04 ng/mL, and the linear range is 0.02-0.09 ng/mL; and the IC50 value of the carbendazim is 3.35 ng/mL, and the linear range of the carbendazim is 0.95 -11.77 ng/mL. The detection method can be used for detecting albendazole in animal food and ca |
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| subjects | CHEMISTRY HETEROCYCLIC COMPOUNDS INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MEASURING METALLURGY ORGANIC CHEMISTRY PEPTIDES PHYSICS TESTING |
| title | Preparation and application of antibody for bispecific recognition of albendazole and carbendazim |
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