Method for preparing high-purity dilinoyl phosphatidylcholine through combination of enzymatic modification and reversed-phase column chromatography separation and product of high-purity dilinoyl phosphatidylcholine
The invention belongs to the field of phospholipid processing, and particularly relates to a method for preparing high-purity dilinoyl phosphatidylcholine through combination of enzymatic modification and reversed-phase column chromatography separation and a product of the high-purity dilinoyl phosp...
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| creator | LIU SIMIN CONG YANXIA ZHANG HAILONG ZHANG WEINONG QI YUTANG |
| description | The invention belongs to the field of phospholipid processing, and particularly relates to a method for preparing high-purity dilinoyl phosphatidylcholine through combination of enzymatic modification and reversed-phase column chromatography separation and a product of the high-purity dilinoyl phosphatidylcholine. According to the method, high-purity phosphatidylcholine (PC) and linoleic acid are used as raw materials, an ultrasonic continuous flow cell crusher is adopted to pre-mix a substrate and lipase before lipase reaction, the reaction product of the system is subjected to acetone desolvation to obtain an acetone insoluble substance rich in dilinoyl phosphatidylcholine, the acetone insoluble substance is added to a chromatographic column with C18 as a stationary phase, and eluting is carried out with low-carbon alcohol or a low-carbon alcohol-water solution to obtain a DLPC product with the purity of more than 95% and the yield of more than 65%. The preparation method disclosed by the invention is mild |
| format | Patent |
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According to the method, high-purity phosphatidylcholine (PC) and linoleic acid are used as raw materials, an ultrasonic continuous flow cell crusher is adopted to pre-mix a substrate and lipase before lipase reaction, the reaction product of the system is subjected to acetone desolvation to obtain an acetone insoluble substance rich in dilinoyl phosphatidylcholine, the acetone insoluble substance is added to a chromatographic column with C18 as a stationary phase, and eluting is carried out with low-carbon alcohol or a low-carbon alcohol-water solution to obtain a DLPC product with the purity of more than 95% and the yield of more than 65%. 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| subjects | ACYCLIC, CARBOCYCLIC OR HETEROCYCLIC COMPOUNDS CONTAININGELEMENTS OTHER THAN CARBON, HYDROGEN, HALOGEN, OXYGEN, NITROGEN,SULFUR, SELENIUM OR TELLURIUM BEER BIOCHEMISTRY CHEMISTRY ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING ORGANIC CHEMISTRY SPIRITS VINEGAR WINE |
| title | Method for preparing high-purity dilinoyl phosphatidylcholine through combination of enzymatic modification and reversed-phase column chromatography separation and product of high-purity dilinoyl phosphatidylcholine |
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