Method for in-vitro amplification of NK immune cells
The invention discloses an NK immune cell in-vitro amplification method. The method comprises heparin anticoagulation conventional blood sampling, peripheral blood lymphocyte blood separation, NK immune cell culture medium preparation, original NK immune cell separation, original NK immune cell cult...
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| creator | CHEN LE GUO MENGYANG MA QING QIU MENGGUANG WANG XIAOLONG |
| description | The invention discloses an NK immune cell in-vitro amplification method. The method comprises heparin anticoagulation conventional blood sampling, peripheral blood lymphocyte blood separation, NK immune cell culture medium preparation, original NK immune cell separation, original NK immune cell culture, original NK immune cell induction and NK immune cell amplification. A culture medium with special components is utilized, a traditional method for slowly culturing the NK immune cells is not needed, the activity of the NK immune cells is improved, the occurrence rate of events such as cell viability weakening and microbial contamination possibly occurring due to long-term in-vitro culture is greatly reduced, an inducer for the cultured original NK immune cells is PMA + ionomycin, T cells are intensively activated, high-concentration cell factors stay in cells in cooperation with a protein transport inhibitor, detection can be easily conducted through antibodies and flow modes, meanwhile, the culture environmen |
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The method comprises heparin anticoagulation conventional blood sampling, peripheral blood lymphocyte blood separation, NK immune cell culture medium preparation, original NK immune cell separation, original NK immune cell culture, original NK immune cell induction and NK immune cell amplification. A culture medium with special components is utilized, a traditional method for slowly culturing the NK immune cells is not needed, the activity of the NK immune cells is improved, the occurrence rate of events such as cell viability weakening and microbial contamination possibly occurring due to long-term in-vitro culture is greatly reduced, an inducer for the cultured original NK immune cells is PMA + ionomycin, T cells are intensively activated, high-concentration cell factors stay in cells in cooperation with a protein transport inhibitor, detection can be easily conducted through antibodies and flow modes, meanwhile, the culture environmen</description><language>chi ; eng</language><subject>BEER ; BIOCHEMISTRY ; CHEMISTRY ; COMPOSITIONS THEREOF ; CULTURE MEDIA ; ENZYMOLOGY ; METALLURGY ; MICROBIOLOGY ; MICROORGANISMS OR ENZYMES ; MUTATION OR GENETIC ENGINEERING ; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS ; SPIRITS ; VINEGAR ; WINE</subject><creationdate>2021</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20210713&DB=EPODOC&CC=CN&NR=113106063A$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,777,882,25545,76296</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20210713&DB=EPODOC&CC=CN&NR=113106063A$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>CHEN LE</creatorcontrib><creatorcontrib>GUO MENGYANG</creatorcontrib><creatorcontrib>MA QING</creatorcontrib><creatorcontrib>QIU MENGGUANG</creatorcontrib><creatorcontrib>WANG XIAOLONG</creatorcontrib><title>Method for in-vitro amplification of NK immune cells</title><description>The invention discloses an NK immune cell in-vitro amplification method. 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A culture medium with special components is utilized, a traditional method for slowly culturing the NK immune cells is not needed, the activity of the NK immune cells is improved, the occurrence rate of events such as cell viability weakening and microbial contamination possibly occurring due to long-term in-vitro culture is greatly reduced, an inducer for the cultured original NK immune cells is PMA + ionomycin, T cells are intensively activated, high-concentration cell factors stay in cells in cooperation with a protein transport inhibitor, detection can be easily conducted through antibodies and flow modes, meanwhile, the culture environmen</abstract><oa>free_for_read</oa></addata></record> |
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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE |
| title | Method for in-vitro amplification of NK immune cells |
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