Preparation of PLGA-PEG-Poly I: C nanoparticles and application of PLGA-PEG-Poly I: C nanoparticles in tuberculosis subunit vaccine

The invention discloses a preparation method of PLGA-PEG-Poly I: C nanoparticles. The preparation method comprises the following steps: forming a PLGA-PEG copolymer from PLGA and NH2-PEG by using a chemical synthesis method, wrapping two mycobacterium tuberculosis fusion proteins ESAT6-Rv2626c and M...

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Hauptverfasser:	HE PU, NIU HONGXIA, LI FEI, TAN DAQUAN, DU XIUFEN, TAN JIYING, ZHU BINGDONG, GONG YANG
Format:	Patent
Sprache:	chi ; eng
Schlagworte:	HUMAN NECESSITIES HYGIENE MEDICAL OR VETERINARY SCIENCE PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS
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Zusammenfassung:	The invention discloses a preparation method of PLGA-PEG-Poly I: C nanoparticles. The preparation method comprises the following steps: forming a PLGA-PEG copolymer from PLGA and NH2-PEG by using a chemical synthesis method, wrapping two mycobacterium tuberculosis fusion proteins ESAT6-Rv2626c and Mtb10.4-HspX (M4 for short) constructed in an experiment in the nanoparticles by using a multiple emulsion solvent evaporation method (W/O/W), modifying by using polydopamine and PolyI: C, and forming the PLGA-PEG-PolyI: C (NP for short) nano-particle tuberculosis subunit vaccine NP/M4 wrapping the mycobacterium tuberculosis fusion protein M4. After a mouse is immunized by adopting the nanoparticle tuberculosis subunit vaccine provided by the invention, after an NP/M4 group is stimulated by HspX protein and Rv2626c protein, the level of IFN-gamma and IL-2 secreted by spleen lymphocytes is obviously higher than that of a PBS group and a BCG group, which indicates that the NP/M4 group can generate IFN-gamma and IL-2 w