Monoclonal antibody against CK8 protein and cell strain, preparation method and application thereof
The invention relates to a monoclonal antibody capable of recognizing a human leukocyte differentiation antigen CK8, a secretory cell strain, a preparation method thereof and application in immunodetection. According to the technical scheme, 340-365 amino acid sequences at the C tail end of CK8 prot...
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| creator | WANG XIAOYA YANG QINGHAI JIANG NINGCHENG FU CHUNHUI CHEN HUILING LIN TING |
| description | The invention relates to a monoclonal antibody capable of recognizing a human leukocyte differentiation antigen CK8, a secretory cell strain, a preparation method thereof and application in immunodetection. According to the technical scheme, 340-365 amino acid sequences at the C tail end of CK8 protein are selected as antigen peptides, and the antigen peptides are coupled with KLH carrier protein to obtain the immunogen; the amino acid sequence of the antigen peptide is shown as SEQID1, a mouse is immunized, and the mouse hybridoma cell strain 14C2 capable of efficiently secreting the anti-CK8 protein monoclonal antibody and the anti-CK8 protein monoclonal antibody secreted by the cell strain are obtained through cell fusion, screening and subcloning. The antibody obtained by the scheme has high specificity and sensitivity, can specifically recognize cells expressing the CK8 protein, and is suitable for immunological detection, especially immunohistochemical detection.
本发明涉及一种可以识别人白细胞分化抗原CK8的单克隆抗体，分泌细胞株、其制备方法 |
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本发明涉及一种可以识别人白细胞分化抗原CK8的单克隆抗体，分泌细胞株、其制备方法</description><subject>BEER</subject><subject>BIOCHEMISTRY</subject><subject>CHEMISTRY</subject><subject>COMPOSITIONS THEREOF</subject><subject>CULTURE MEDIA</subject><subject>ENZYMOLOGY</subject><subject>INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES</subject><subject>MEASURING</subject><subject>METALLURGY</subject><subject>MICROBIOLOGY</subject><subject>MICROORGANISMS OR ENZYMES</subject><subject>MUTATION OR GENETIC ENGINEERING</subject><subject>ORGANIC CHEMISTRY</subject><subject>PEPTIDES</subject><subject>PHYSICS</subject><subject>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</subject><subject>SPIRITS</subject><subject>TESTING</subject><subject>VINEGAR</subject><subject>WINE</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>2021</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNqNyjEKAjEQheE0FqLeYewVjFqspQRFEK3slzE76wbiTEim8fYG9QBWD973j42_CIuPwhgBWcNduhfgAwMXBXduIGVRClyxA08xQtFcdVGBEmbUIAxP0kG6T4MpxeC_tw6USfqpGfUYC81-OzHz4-HmTktK0lJJ6IlJW3e1dr3brqxt9pt_mjd69j5E</recordid><startdate>20210611</startdate><enddate>20210611</enddate><creator>WANG XIAOYA</creator><creator>YANG QINGHAI</creator><creator>JIANG NINGCHENG</creator><creator>FU CHUNHUI</creator><creator>CHEN HUILING</creator><creator>LIN TING</creator><scope>EVB</scope></search><sort><creationdate>20210611</creationdate><title>Monoclonal antibody against CK8 protein and cell strain, preparation method and application thereof</title><author>WANG XIAOYA ; YANG QINGHAI ; JIANG NINGCHENG ; FU CHUNHUI ; CHEN HUILING ; LIN TING</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_CN112940118A3</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>chi ; eng</language><creationdate>2021</creationdate><topic>BEER</topic><topic>BIOCHEMISTRY</topic><topic>CHEMISTRY</topic><topic>COMPOSITIONS THEREOF</topic><topic>CULTURE MEDIA</topic><topic>ENZYMOLOGY</topic><topic>INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES</topic><topic>MEASURING</topic><topic>METALLURGY</topic><topic>MICROBIOLOGY</topic><topic>MICROORGANISMS OR ENZYMES</topic><topic>MUTATION OR GENETIC ENGINEERING</topic><topic>ORGANIC CHEMISTRY</topic><topic>PEPTIDES</topic><topic>PHYSICS</topic><topic>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</topic><topic>SPIRITS</topic><topic>TESTING</topic><topic>VINEGAR</topic><topic>WINE</topic><toplevel>online_resources</toplevel><creatorcontrib>WANG XIAOYA</creatorcontrib><creatorcontrib>YANG QINGHAI</creatorcontrib><creatorcontrib>JIANG NINGCHENG</creatorcontrib><creatorcontrib>FU CHUNHUI</creatorcontrib><creatorcontrib>CHEN HUILING</creatorcontrib><creatorcontrib>LIN TING</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>WANG XIAOYA</au><au>YANG QINGHAI</au><au>JIANG NINGCHENG</au><au>FU CHUNHUI</au><au>CHEN HUILING</au><au>LIN TING</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>Monoclonal antibody against CK8 protein and cell strain, preparation method and application thereof</title><date>2021-06-11</date><risdate>2021</risdate><abstract>The invention relates to a monoclonal antibody capable of recognizing a human leukocyte differentiation antigen CK8, a secretory cell strain, a preparation method thereof and application in immunodetection. According to the technical scheme, 340-365 amino acid sequences at the C tail end of CK8 protein are selected as antigen peptides, and the antigen peptides are coupled with KLH carrier protein to obtain the immunogen; the amino acid sequence of the antigen peptide is shown as SEQID1, a mouse is immunized, and the mouse hybridoma cell strain 14C2 capable of efficiently secreting the anti-CK8 protein monoclonal antibody and the anti-CK8 protein monoclonal antibody secreted by the cell strain are obtained through cell fusion, screening and subcloning. The antibody obtained by the scheme has high specificity and sensitivity, can specifically recognize cells expressing the CK8 protein, and is suitable for immunological detection, especially immunohistochemical detection.
本发明涉及一种可以识别人白细胞分化抗原CK8的单克隆抗体，分泌细胞株、其制备方法</abstract><oa>free_for_read</oa></addata></record> |
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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MEASURING METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING ORGANIC CHEMISTRY PEPTIDES PHYSICS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS TESTING VINEGAR WINE |
| title | Monoclonal antibody against CK8 protein and cell strain, preparation method and application thereof |
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