Double-stranded nucleic acid cyclization method, methylation sequencing library construction method and kit
The invention discloses a nucleic acid double-chain cyclization method, a methylation sequencing library construction method and a kit. The nucleic acid double-strand cyclization method comprises the following steps: providing a forward primer and a reverse primer, wherein each of the forward primer...
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| creator | YANG LINFENG HE CHANGSHOU TONG YIQIN GAO QIANG WANG JUAN TANG CHONG |
| description | The invention discloses a nucleic acid double-chain cyclization method, a methylation sequencing library construction method and a kit. The nucleic acid double-strand cyclization method comprises the following steps: providing a forward primer and a reverse primer, wherein each of the forward primer and the reverse primer comprises a U basic group, the nucleotide number difference of the upstream sequences of the 5'ends of the U basic groups of the forward primer and the reverse primer is N, and the upstream sequences of the 5 'ends of the U basic groups of the forward primer and the reverse primer are at least partially and reversely complementary; amplifying the nucleic acid fragment by using the primer pair to obtain an amplification product; a USER enzyme is used for cutting off U basic groups in the amplification product, then a mononucleotide gap is formed, chain dissociation occurs to the residual nucleotide sequence from the gap to the 5'end due to the unstable structure, and then partially complement |
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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMBINATORIAL CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY LIBRARIES, e.g. CHEMICAL LIBRARIES, IN SILICOLIBRARIES MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE |
| title | Double-stranded nucleic acid cyclization method, methylation sequencing library construction method and kit |
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