Cell line for detecting activity of human interferon-kappa and construction and detection method thereof
The invention discloses a cell line for detecting the activity of human interferon-kappa and a construction and detection method thereof. The cell line is obtained by transfecting eukaryotic cells with ISRE (interferon-sensitive response element) luciferase reporter genes and stably screening monocl...
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| creator | PENG JIXIAN CHAI HUI YU HAIQIN GUO LINGHUA QIAN WENZHENG YAN YUNQIU |
| description | The invention discloses a cell line for detecting the activity of human interferon-kappa and a construction and detection method thereof. The cell line is obtained by transfecting eukaryotic cells with ISRE (interferon-sensitive response element) luciferase reporter genes and stably screening monoclonal cell lines. The cell line constructed by the invention is easy to culture and amplify and good in passage stability, and has the advantages of high sensitivity, short detection time, completion within 4-6 hours, and capability of greatly improving the activity release efficiency of the human interferon-kappa in the production process.
本发明公开了一种用于人干扰素-κ活性检测的细胞系及其构建和检测方法。本发明的细胞系通过使用ISRE(干扰素敏感响应元件)荧光素酶报告基因转染真核细胞，通过稳定筛选单克隆细胞系获得。本发明构建的细胞系易于培养扩增和传代稳定性好，且检测人干扰素-κ的活性具有灵敏度高、检测时间短、4-6小时内即可完成、能够大幅提高人干扰素-κ在生产过程制造中的活性放行效率的优点。 |
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本发明公开了一种用于人干扰素-κ活性检测的细胞系及其构建和检测方法。本发明的细胞系通过使用ISRE(干扰素敏感响应元件)荧光素酶报告基因转染真核细胞，通过稳定筛选单克隆细胞系获得。本发明构建的细胞系易于培养扩增和传代稳定性好，且检测人干扰素-κ的活性具有灵敏度高、检测时间短、4-6小时内即可完成、能够大幅提高人干扰素-κ在生产过程制造中的活性放行效率的优点。</description><language>chi ; eng</language><subject>BEER ; BIOCHEMISTRY ; CHEMISTRY ; COMPOSITIONS OR TEST PAPERS THEREFOR ; COMPOSITIONS THEREOF ; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ; CULTURE MEDIA ; ENZYMOLOGY ; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES ; MEASURING ; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS ; METALLURGY ; MICROBIOLOGY ; MICROORGANISMS OR ENZYMES ; MUTATION OR GENETIC ENGINEERING ; PHYSICS ; PROCESSES OF PREPARING SUCH COMPOSITIONS ; PROCESSES USING MICROORGANISMS ; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS ; SPIRITS ; TESTING ; VINEGAR ; WINE</subject><creationdate>2021</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20210507&DB=EPODOC&CC=CN&NR=112760291A$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,780,885,25564,76547</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20210507&DB=EPODOC&CC=CN&NR=112760291A$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>PENG JIXIAN</creatorcontrib><creatorcontrib>CHAI HUI</creatorcontrib><creatorcontrib>YU HAIQIN</creatorcontrib><creatorcontrib>GUO LINGHUA</creatorcontrib><creatorcontrib>QIAN WENZHENG</creatorcontrib><creatorcontrib>YAN YUNQIU</creatorcontrib><title>Cell line for detecting activity of human interferon-kappa and construction and detection method thereof</title><description>The invention discloses a cell line for detecting the activity of human interferon-kappa and a construction and detection method thereof. The cell line is obtained by transfecting eukaryotic cells with ISRE (interferon-sensitive response element) luciferase reporter genes and stably screening monoclonal cell lines. The cell line constructed by the invention is easy to culture and amplify and good in passage stability, and has the advantages of high sensitivity, short detection time, completion within 4-6 hours, and capability of greatly improving the activity release efficiency of the human interferon-kappa in the production process.
本发明公开了一种用于人干扰素-κ活性检测的细胞系及其构建和检测方法。本发明的细胞系通过使用ISRE(干扰素敏感响应元件)荧光素酶报告基因转染真核细胞，通过稳定筛选单克隆细胞系获得。本发明构建的细胞系易于培养扩增和传代稳定性好，且检测人干扰素-κ的活性具有灵敏度高、检测时间短、4-6小时内即可完成、能够大幅提高人干扰素-κ在生产过程制造中的活性放行效率的优点。</description><subject>BEER</subject><subject>BIOCHEMISTRY</subject><subject>CHEMISTRY</subject><subject>COMPOSITIONS OR TEST PAPERS THEREFOR</subject><subject>COMPOSITIONS THEREOF</subject><subject>CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES</subject><subject>CULTURE MEDIA</subject><subject>ENZYMOLOGY</subject><subject>INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES</subject><subject>MEASURING</subject><subject>MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS</subject><subject>METALLURGY</subject><subject>MICROBIOLOGY</subject><subject>MICROORGANISMS OR ENZYMES</subject><subject>MUTATION OR GENETIC ENGINEERING</subject><subject>PHYSICS</subject><subject>PROCESSES OF PREPARING SUCH COMPOSITIONS</subject><subject>PROCESSES USING MICROORGANISMS</subject><subject>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</subject><subject>SPIRITS</subject><subject>TESTING</subject><subject>VINEGAR</subject><subject>WINE</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>2021</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNqNjEEKwjAQRbtxIeodxgMUbAXFpQTFlSv3JbQ_JpjOhHQqeHuL9ACuHu_z-MvCG8RIMTDISaYOilYDP8lOeAf9kDjyY2-ZAiuyQxYuXzYlS5Y7aoUHzeMUC_-G-WGyHuqlI_XIELcuFs7GAZuZq2J7vTzMrUSSBkOyLRjamHtV1cfDrj5V5_0_zRcXykCe</recordid><startdate>20210507</startdate><enddate>20210507</enddate><creator>PENG JIXIAN</creator><creator>CHAI HUI</creator><creator>YU HAIQIN</creator><creator>GUO LINGHUA</creator><creator>QIAN WENZHENG</creator><creator>YAN YUNQIU</creator><scope>EVB</scope></search><sort><creationdate>20210507</creationdate><title>Cell line for detecting activity of human interferon-kappa and construction and detection method thereof</title><author>PENG JIXIAN ; CHAI HUI ; YU HAIQIN ; GUO LINGHUA ; QIAN WENZHENG ; YAN YUNQIU</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_CN112760291A3</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>chi ; eng</language><creationdate>2021</creationdate><topic>BEER</topic><topic>BIOCHEMISTRY</topic><topic>CHEMISTRY</topic><topic>COMPOSITIONS OR TEST PAPERS THEREFOR</topic><topic>COMPOSITIONS THEREOF</topic><topic>CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES</topic><topic>CULTURE MEDIA</topic><topic>ENZYMOLOGY</topic><topic>INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES</topic><topic>MEASURING</topic><topic>MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS</topic><topic>METALLURGY</topic><topic>MICROBIOLOGY</topic><topic>MICROORGANISMS OR ENZYMES</topic><topic>MUTATION OR GENETIC ENGINEERING</topic><topic>PHYSICS</topic><topic>PROCESSES OF PREPARING SUCH COMPOSITIONS</topic><topic>PROCESSES USING MICROORGANISMS</topic><topic>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</topic><topic>SPIRITS</topic><topic>TESTING</topic><topic>VINEGAR</topic><topic>WINE</topic><toplevel>online_resources</toplevel><creatorcontrib>PENG JIXIAN</creatorcontrib><creatorcontrib>CHAI HUI</creatorcontrib><creatorcontrib>YU HAIQIN</creatorcontrib><creatorcontrib>GUO LINGHUA</creatorcontrib><creatorcontrib>QIAN WENZHENG</creatorcontrib><creatorcontrib>YAN YUNQIU</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>PENG JIXIAN</au><au>CHAI HUI</au><au>YU HAIQIN</au><au>GUO LINGHUA</au><au>QIAN WENZHENG</au><au>YAN YUNQIU</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>Cell line for detecting activity of human interferon-kappa and construction and detection method thereof</title><date>2021-05-07</date><risdate>2021</risdate><abstract>The invention discloses a cell line for detecting the activity of human interferon-kappa and a construction and detection method thereof. The cell line is obtained by transfecting eukaryotic cells with ISRE (interferon-sensitive response element) luciferase reporter genes and stably screening monoclonal cell lines. The cell line constructed by the invention is easy to culture and amplify and good in passage stability, and has the advantages of high sensitivity, short detection time, completion within 4-6 hours, and capability of greatly improving the activity release efficiency of the human interferon-kappa in the production process.
本发明公开了一种用于人干扰素-κ活性检测的细胞系及其构建和检测方法。本发明的细胞系通过使用ISRE(干扰素敏感响应元件)荧光素酶报告基因转染真核细胞，通过稳定筛选单克隆细胞系获得。本发明构建的细胞系易于培养扩增和传代稳定性好，且检测人干扰素-κ的活性具有灵敏度高、检测时间短、4-6小时内即可完成、能够大幅提高人干扰素-κ在生产过程制造中的活性放行效率的优点。</abstract><oa>free_for_read</oa></addata></record> |
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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MEASURING MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PHYSICS PROCESSES OF PREPARING SUCH COMPOSITIONS PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS TESTING VINEGAR WINE |
| title | Cell line for detecting activity of human interferon-kappa and construction and detection method thereof |
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