Immunoregulatory stem cell IDO1 activity detection method meeting product release requirements

The invention relates to an immunomodulatory stem cell indoleamine 2, 3-dioxygenase 1 (IDO1) activity detection method meeting product release requirements. The invention provides an inspection method which is normative and easy to standardize and can be used for characterizing and releasing the immunoregulation function of the mesenchymal stem cell type cell therapy products, so that clinical immunoregulation effect evaluation is preposed, the success rate of clinical tests is increased, the risk of subjects is reduced, and mesenchymal stem cells are guided to prepare medicines. According to the invention, homogeneous reference cells, namely working reference cells, are prepared, and a relative biological activity detection method is established, so that standard exceeding or incredible detection results caused by system errors can be eliminated. 本发明涉及满足产品放行要求的免疫调节干细胞吲哚胺2,3-双加氧酶1(IDO1)活性检测方法。本发明提供了一种规范的、易于标准化的、能够用于表征和放行间充质干细胞类细胞治疗产品的免疫调控功能的检验方法，从而将临床免疫调节效应评价前置，提高临床试验的成功率，降低受试者风险，指导间充质干细胞成药。本发明制备了同质参考品细胞即工作参考