Method for determining content of hydroxyl-alpha-sanshool
The invention discloses a method for determining the content of hydroxyl-alpha-sanshool. The method comprises the following steps: heating and blow-drying a to-be-determined sample by using inert gas; redissolving with an acetonitrile aqueous solution, and oscillating to obtain an analysis sample; a...
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| creator | LU GUOCAI YAN YUWEN LENG MINGHONG YE SHUANGSHUANG ZONG YING XIA YUYE |
| description | The invention discloses a method for determining the content of hydroxyl-alpha-sanshool. The method comprises the following steps: heating and blow-drying a to-be-determined sample by using inert gas; redissolving with an acetonitrile aqueous solution, and oscillating to obtain an analysis sample; and combining the HPLC-MS to determine and analyze the sample. According to the method, simple and rapid nitrogen blow-drying is adopted for pretreatment, organic reagents such as ethyl acetate interfering with hydroxyl-alpha-sanshool content determination are removed, and interfering substances are separated by using a chromatographic column, so that the measurement result is more accurate. Meanwhile, the method has the advantages of high sensitivity, accurate measurement and simple and rapid operation.
本发明公开了一种测定羟基-α-山椒素含量的方法，包括：使用惰性气体加热吹干待测样品；用乙腈水溶液复溶后震荡得到分析样品；HPLC-MS联用测定分析样品。本发明采用简单快速的氮气吹干进行前处理，去除干扰羟基-α-山椒素含量测定的乙酸乙酯等有机试剂，并利用色谱柱分离干扰物质，使测量结果更精确。同时还具有灵敏度高，测量精确，且操作简单快速的优点。 |
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本发明公开了一种测定羟基-α-山椒素含量的方法，包括：使用惰性气体加热吹干待测样品；用乙腈水溶液复溶后震荡得到分析样品；HPLC-MS联用测定分析样品。本发明采用简单快速的氮气吹干进行前处理，去除干扰羟基-α-山椒素含量测定的乙酸乙酯等有机试剂，并利用色谱柱分离干扰物质，使测量结果更精确。同时还具有灵敏度高，测量精确，且操作简单快速的优点。</description><language>chi ; eng</language><subject>INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES ; MEASURING ; PHYSICS ; TESTING</subject><creationdate>2021</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20210420&DB=EPODOC&CC=CN&NR=112684068A$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,780,885,25564,76547</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20210420&DB=EPODOC&CC=CN&NR=112684068A$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>LU GUOCAI</creatorcontrib><creatorcontrib>YAN YUWEN</creatorcontrib><creatorcontrib>LENG MINGHONG</creatorcontrib><creatorcontrib>YE SHUANGSHUANG</creatorcontrib><creatorcontrib>ZONG YING</creatorcontrib><creatorcontrib>XIA YUYE</creatorcontrib><title>Method for determining content of hydroxyl-alpha-sanshool</title><description>The invention discloses a method for determining the content of hydroxyl-alpha-sanshool. The method comprises the following steps: heating and blow-drying a to-be-determined sample by using inert gas; redissolving with an acetonitrile aqueous solution, and oscillating to obtain an analysis sample; and combining the HPLC-MS to determine and analyze the sample. According to the method, simple and rapid nitrogen blow-drying is adopted for pretreatment, organic reagents such as ethyl acetate interfering with hydroxyl-alpha-sanshool content determination are removed, and interfering substances are separated by using a chromatographic column, so that the measurement result is more accurate. Meanwhile, the method has the advantages of high sensitivity, accurate measurement and simple and rapid operation.
本发明公开了一种测定羟基-α-山椒素含量的方法，包括：使用惰性气体加热吹干待测样品；用乙腈水溶液复溶后震荡得到分析样品；HPLC-MS联用测定分析样品。本发明采用简单快速的氮气吹干进行前处理，去除干扰羟基-α-山椒素含量测定的乙酸乙酯等有机试剂，并利用色谱柱分离干扰物质，使测量结果更精确。同时还具有灵敏度高，测量精确，且操作简单快速的优点。</description><subject>INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES</subject><subject>MEASURING</subject><subject>PHYSICS</subject><subject>TESTING</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>2021</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNrjZLD0TS3JyE9RSMsvUkhJLUktys3My8xLV0jOzytJzStRyE9TyKhMKcqvqMzRTcwpyEjULU7MK87Iz8_hYWBNS8wpTuWF0twMim6uIc4euqkF-fGpxQWJyal5qSXxzn6GhkZmFiYGZhaOxsSoAQBsQC8g</recordid><startdate>20210420</startdate><enddate>20210420</enddate><creator>LU GUOCAI</creator><creator>YAN YUWEN</creator><creator>LENG MINGHONG</creator><creator>YE SHUANGSHUANG</creator><creator>ZONG YING</creator><creator>XIA YUYE</creator><scope>EVB</scope></search><sort><creationdate>20210420</creationdate><title>Method for determining content of hydroxyl-alpha-sanshool</title><author>LU GUOCAI ; YAN YUWEN ; LENG MINGHONG ; YE SHUANGSHUANG ; ZONG YING ; XIA YUYE</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_CN112684068A3</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>chi ; eng</language><creationdate>2021</creationdate><topic>INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES</topic><topic>MEASURING</topic><topic>PHYSICS</topic><topic>TESTING</topic><toplevel>online_resources</toplevel><creatorcontrib>LU GUOCAI</creatorcontrib><creatorcontrib>YAN YUWEN</creatorcontrib><creatorcontrib>LENG MINGHONG</creatorcontrib><creatorcontrib>YE SHUANGSHUANG</creatorcontrib><creatorcontrib>ZONG YING</creatorcontrib><creatorcontrib>XIA YUYE</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>LU GUOCAI</au><au>YAN YUWEN</au><au>LENG MINGHONG</au><au>YE SHUANGSHUANG</au><au>ZONG YING</au><au>XIA YUYE</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>Method for determining content of hydroxyl-alpha-sanshool</title><date>2021-04-20</date><risdate>2021</risdate><abstract>The invention discloses a method for determining the content of hydroxyl-alpha-sanshool. The method comprises the following steps: heating and blow-drying a to-be-determined sample by using inert gas; redissolving with an acetonitrile aqueous solution, and oscillating to obtain an analysis sample; and combining the HPLC-MS to determine and analyze the sample. According to the method, simple and rapid nitrogen blow-drying is adopted for pretreatment, organic reagents such as ethyl acetate interfering with hydroxyl-alpha-sanshool content determination are removed, and interfering substances are separated by using a chromatographic column, so that the measurement result is more accurate. Meanwhile, the method has the advantages of high sensitivity, accurate measurement and simple and rapid operation.
本发明公开了一种测定羟基-α-山椒素含量的方法，包括：使用惰性气体加热吹干待测样品；用乙腈水溶液复溶后震荡得到分析样品；HPLC-MS联用测定分析样品。本发明采用简单快速的氮气吹干进行前处理，去除干扰羟基-α-山椒素含量测定的乙酸乙酯等有机试剂，并利用色谱柱分离干扰物质，使测量结果更精确。同时还具有灵敏度高，测量精确，且操作简单快速的优点。</abstract><oa>free_for_read</oa></addata></record> |
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| title | Method for determining content of hydroxyl-alpha-sanshool |
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