Preparation method of recombinant NcoI restriction endonuclease
The invention provides a preparation method of a recombinant NcoI restriction enzyme, which comprises the following steps: 1, synthesizing NcoIM and NcoI coding genes, and respectively constructing the NcoIM and NcoI coding genes into corresponding vectors to obtain a recombinant vector with the Nco...
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| creator | SHAN YONGCHAO LI JIANHUI YU BOWEN |
| description | The invention provides a preparation method of a recombinant NcoI restriction enzyme, which comprises the following steps: 1, synthesizing NcoIM and NcoI coding genes, and respectively constructing the NcoIM and NcoI coding genes into corresponding vectors to obtain a recombinant vector with the NcoIM gene and an NcoI protein expression plasmid fused with a purification tag; 2, transferring the recombinant vector with the NcoIM gene into escherichia coli to obtain recombinant bacteria with substrate NcoIM expression; 3, preparing a recombinant bacterium competent cell with substrate NcoIM expression; 4, transferring the NcoI protein expression plasmid fused with the purification tag into a recombinant bacterium competent cell with substrate NcoIM expression to obtain positive monoclone, and culturing to express the NcoI restriction endonuclease; and 5, purifying the NcoI restriction endonuclease. Through the method provided by the invention, a large amount of NcoI restriction endonucleases can be obtained, an |
| format | Patent |
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Through the method provided by the invention, a large amount of NcoI restriction endonucleases can be obtained, an</description><language>chi ; eng</language><subject>BEER ; BIOCHEMISTRY ; CHEMISTRY ; COMPOSITIONS THEREOF ; CULTURE MEDIA ; ENZYMOLOGY ; METALLURGY ; MICROBIOLOGY ; MICROORGANISMS OR ENZYMES ; MUTATION OR GENETIC ENGINEERING ; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS ; SPIRITS ; VINEGAR ; WINE</subject><creationdate>2021</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20210416&DB=EPODOC&CC=CN&NR=112662647A$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,780,885,25564,76547</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20210416&DB=EPODOC&CC=CN&NR=112662647A$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>SHAN YONGCHAO</creatorcontrib><creatorcontrib>LI JIANHUI</creatorcontrib><creatorcontrib>YU BOWEN</creatorcontrib><title>Preparation method of recombinant NcoI restriction endonuclease</title><description>The invention provides a preparation method of a recombinant NcoI restriction enzyme, which comprises the following steps: 1, synthesizing NcoIM and NcoI coding genes, and respectively constructing the NcoIM and NcoI coding genes into corresponding vectors to obtain a recombinant vector with the NcoIM gene and an NcoI protein expression plasmid fused with a purification tag; 2, transferring the recombinant vector with the NcoIM gene into escherichia coli to obtain recombinant bacteria with substrate NcoIM expression; 3, preparing a recombinant bacterium competent cell with substrate NcoIM expression; 4, transferring the NcoI protein expression plasmid fused with the purification tag into a recombinant bacterium competent cell with substrate NcoIM expression to obtain positive monoclone, and culturing to express the NcoI restriction endonuclease; and 5, purifying the NcoI restriction endonuclease. 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Through the method provided by the invention, a large amount of NcoI restriction endonucleases can be obtained, an</abstract><oa>free_for_read</oa></addata></record> |
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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE |
| title | Preparation method of recombinant NcoI restriction endonuclease |
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