Purification method of recombinant human GLP-1-Fc fusion protein
The invention discloses a purification method of recombinant human GLP-1-Fc fusion protein, which comprises the steps of carrying out coarse purification on a sample containing the recombinant human GLP-1-Fc fusion protein and then further purifying by adopting a preparative chromatography; the puri...
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| creator | SHI CHUANCHAO HAO XIANGHUI XIE FUSHENG LIU HAIFENG NIE HONGXIA HUANG JING ZHU RU |
| description | The invention discloses a purification method of recombinant human GLP-1-Fc fusion protein, which comprises the steps of carrying out coarse purification on a sample containing the recombinant human GLP-1-Fc fusion protein and then further purifying by adopting a preparative chromatography; the purification method of the preparative chromatography comprises the following steps: (1) carrying out anion exchange chromatography on a primarily purified product; (2) eluting the impure protein adsorbed on the column in a gradient elution manner; and (3) collecting a main peak by adopting an isocratic elution mode to obtain a target product. The purification method is simple and feasible in process, the purified sample is high in purity and yield, the purity of the crude sample can be improved to 99.6% or above from 95.0% in one step, host protein can be removed, the yield of the purified sample is stable, the sample loading amount of the crude sample is large, the purification cost is greatly reduced, the process is |
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| title | Purification method of recombinant human GLP-1-Fc fusion protein |
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