Primer, probe and application for identifying CDV (canine distemper virus) wild strain and vaccine strain

The invention provides a primer, a probe and application for identifying a CDV (canine distemper virus) wild strain and a vaccine strain. The primer comprises an upstream primer with a nucleotide sequence shown as SEQ ID NO. 1, a downstream primer with a nucleotide sequence shown as SEQ ID NO. 2, a vaccine strain probe with a nucleotide sequence shown as SEQ ID NO. 3 and a wild strain probe with a nucleotide sequence shown as SEQ ID NO. 4. A composite fluorescent quantitative PCR method constructed by the primer and the probe can be used for quickly and effectively identifying the CDV wild strain and the vaccine strain, and has the characteristics of high sensitivity, high specificity and high repeatability. 本发明提供了一种鉴别犬瘟热病毒野毒株和疫苗株的引物、探针及应用，包括核苷酸序列如SEQIDNO.1所示的上游引物、核苷酸序列如SEQIDNO.2所示的下游引物；和核苷酸序列如SEQIDNO.3所示的疫苗株探针及核苷酸序列如SEQIDNO.4所示的野毒株探针。本发明引物探针构建的复合荧光定量PCR方法能快速、有效的鉴别犬瘟热病毒(CDV)野毒株和疫苗株，具有灵敏度高、特异性强、重复性好的特点。