Spinal cord OLIG2<+> nerve progenitor cell induction and self-renewal culture system, induction method and application
The invention relates to a spinal cord OLIG2 nerve progenitor cell induction and self-renewal culture system, an induction method and application. The culture system comprises a basic culture medium, an induced differentiation factor and an in-vitro maintenance factor, wherein the induced differenti...
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| creator | SUN PINGXIN YUAN YUAN LI WENLIN LYU ZHUMAN ZHANG GUANYU |
| description | The invention relates to a spinal cord OLIG2 nerve progenitor cell induction and self-renewal culture system, an induction method and application. The culture system comprises a basic culture medium, an induced differentiation factor and an in-vitro maintenance factor, wherein the induced differentiation factor and the in-vitro maintenance factor are combined with the basic culture medium for use; the induced differentiation factor at least comprises a 0.01-50 microns of GSK3 inhibitor, a 0.01-500 microns of TGF beta inhibitor and a 0.01-500 microns of BMP inhibitor; and the in-vitro maintenance factor comprises a 0.01-50 microns of GSK3 inhibitor, a 0.01-500 microns of TGF beta inhibitor, 0.01-5000 ng/mL of Sonic Hedgehog, a 0.01-20 microns of Smoothened agonist, a 0.01-500ng/mL of LEGF family growth factor, a 0.01-500 ng/mL of FGF family growth factor, a 0.01-500 microns of BMP inhibitor, a 0.01-50 microns of JAK1/2 inhibitor, a 0.01-10000nM of Src kinase inhibitor and a 0.01-50 microns of RetinoicAcid. |
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The culture system comprises a basic culture medium, an induced differentiation factor and an in-vitro maintenance factor, wherein the induced differentiation factor and the in-vitro maintenance factor are combined with the basic culture medium for use; the induced differentiation factor at least comprises a 0.01-50 microns of GSK3 inhibitor, a 0.01-500 microns of TGF beta inhibitor and a 0.01-500 microns of BMP inhibitor; and the in-vitro maintenance factor comprises a 0.01-50 microns of GSK3 inhibitor, a 0.01-500 microns of TGF beta inhibitor, 0.01-5000 ng/mL of Sonic Hedgehog, a 0.01-20 microns of Smoothened agonist, a 0.01-500ng/mL of LEGF family growth factor, a 0.01-500 ng/mL of FGF family growth factor, a 0.01-500 microns of BMP inhibitor, a 0.01-50 microns of JAK1/2 inhibitor, a 0.01-10000nM of Src kinase inhibitor and a 0.01-50 microns of RetinoicAcid.</abstract><oa>free_for_read</oa></addata></record> |
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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY HUMAN NECESSITIES HYGIENE MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS MEDICAL OR VETERINARY SCIENCE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES PROCESSES OF PREPARING SUCH COMPOSITIONS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS SPIRITS VINEGAR WINE |
| title | Spinal cord OLIG2<+> nerve progenitor cell induction and self-renewal culture system, induction method and application |
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