Preparation method of cell vaccine antigen
The invention discloses a preparation method of a cell vaccine antigen, which comprises the steps of healthy cell passage, virus inoculation and liquid replacement, antigen harvesting and the like; during cell passage and digestion, an oscillator is used for replacing manual bottle throwing, so that...
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| creator | HUANG SHIXIAO ZHENG JINGMIN ZHANG XIAOLAN WU JINFEI CHEN SHUYA CAI YANHONG YE ZHIHAI |
| description | The invention discloses a preparation method of a cell vaccine antigen, which comprises the steps of healthy cell passage, virus inoculation and liquid replacement, antigen harvesting and the like; during cell passage and digestion, an oscillator is used for replacing manual bottle throwing, so that the labor intensity can be greatly reduced; during the liquid replacement, a plurality of glass bottles filled with fresh maintenance liquid are connected in series, and the maintenance liquid in the glass bottles is pumped into the cell culture bottles through the peristaltic pump, so that the liquid replacement process is semi-automatic, the number of operators can be reduced, the labor intensity in the production process can be reduced, the production efficiency can be improved, and the pollution rate of semi-finished products can be reduced; when the antigen is harvested, cells in the cell culture bottle are broken mainly through ultrasonic energy conversion method, thereby scraping thevaccines, so that the de |
| format | Patent |
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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE |
| title | Preparation method of cell vaccine antigen |
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