TLN-58 and hLYZ gene fusion protein and antibacterial activity and application thereof
The invention relates to an hLYZ-TLN-58 gene fusion protein. The fusion protein is constructed by the following steps of (1) optimizing to obtain an hLYZ-TLN-58 gene SEQ ID NO.9 which is efficiently expressed in a mouse body according to a TLN-58 gene base sequence GI: AP02750 and an hLYZ gene stand...
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| creator | JIN TIANMING CHEN TING WANG XIAOYUE ZHAO WEI LAN ZHAOYU LI DAN FAN ZHENWEI WU JINGQIAO HE JINGWEN YAO JINGLI |
| description | The invention relates to an hLYZ-TLN-58 gene fusion protein. The fusion protein is constructed by the following steps of (1) optimizing to obtain an hLYZ-TLN-58 gene SEQ ID NO.9 which is efficiently expressed in a mouse body according to a TLN-58 gene base sequence GI: AP02750 and an hLYZ gene standard sequence GI: NC_000012.10; (2) carrying out PCR amplification on the hLYZ-TLN-58 gene; and (3) expressing and purifying the recombinant prokaryotic expression vector pGEX-4T-1-hLYZ-TLN-58 to obtain the hLYZ-TLN-58 gene fusion protein. According to the fusion protein and antibacterial activity and the application thereof, the protein expressed by the recombinant prokaryotic plasmid constructed shows good bacteriostatic activity on an experimental strain, and a new treatment thought and experimental basis for clinical prevention and treatment of bacterial diseases is provided.
本发明涉及一种hLYZ-TLN-58基因融合蛋白，构建步骤如下：⑴根据TLN-58基因碱基序列GI：AP02750和hLYZ基因标准序列GI：NC_000012.10，优化得到在小鼠体内高效表达的hLYZ-TLN-58基因SEQ ID NO.9；⑵PCR扩增hLYZ-TLN- |
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本发明涉及一种hLYZ-TLN-58基因融合蛋白，构建步骤如下：⑴根据TLN-58基因碱基序列GI：AP02750和hLYZ基因标准序列GI：NC_000012.10，优化得到在小鼠体内高效表达的hLYZ-TLN-58基因SEQ ID NO.9；⑵PCR扩增hLYZ-TLN-</description><language>chi ; eng</language><subject>BEER ; BIOCHEMISTRY ; CHEMISTRY ; COMPOSITIONS THEREOF ; CULTURE MEDIA ; ENZYMOLOGY ; HUMAN NECESSITIES ; HYGIENE ; MEDICAL OR VETERINARY SCIENCE ; METALLURGY ; MICROBIOLOGY ; MICROORGANISMS OR ENZYMES ; MUTATION OR GENETIC ENGINEERING ; ORGANIC CHEMISTRY ; PEPTIDES ; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES ; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS ; SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS ; SPIRITS ; VINEGAR ; WINE</subject><creationdate>2021</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20210309&DB=EPODOC&CC=CN&NR=112457409A$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,776,881,25542,76290</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20210309&DB=EPODOC&CC=CN&NR=112457409A$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>JIN TIANMING</creatorcontrib><creatorcontrib>CHEN TING</creatorcontrib><creatorcontrib>WANG XIAOYUE</creatorcontrib><creatorcontrib>ZHAO WEI</creatorcontrib><creatorcontrib>LAN ZHAOYU</creatorcontrib><creatorcontrib>LI DAN</creatorcontrib><creatorcontrib>FAN ZHENWEI</creatorcontrib><creatorcontrib>WU JINGQIAO</creatorcontrib><creatorcontrib>HE JINGWEN</creatorcontrib><creatorcontrib>YAO JINGLI</creatorcontrib><title>TLN-58 and hLYZ gene fusion protein and antibacterial activity and application thereof</title><description>The invention relates to an hLYZ-TLN-58 gene fusion protein. The fusion protein is constructed by the following steps of (1) optimizing to obtain an hLYZ-TLN-58 gene SEQ ID NO.9 which is efficiently expressed in a mouse body according to a TLN-58 gene base sequence GI: AP02750 and an hLYZ gene standard sequence GI: NC_000012.10; (2) carrying out PCR amplification on the hLYZ-TLN-58 gene; and (3) expressing and purifying the recombinant prokaryotic expression vector pGEX-4T-1-hLYZ-TLN-58 to obtain the hLYZ-TLN-58 gene fusion protein. According to the fusion protein and antibacterial activity and the application thereof, the protein expressed by the recombinant prokaryotic plasmid constructed shows good bacteriostatic activity on an experimental strain, and a new treatment thought and experimental basis for clinical prevention and treatment of bacterial diseases is provided.
本发明涉及一种hLYZ-TLN-58基因融合蛋白，构建步骤如下：⑴根据TLN-58基因碱基序列GI：AP02750和hLYZ基因标准序列GI：NC_000012.10，优化得到在小鼠体内高效表达的hLYZ-TLN-58基因SEQ ID NO.9；⑵PCR扩增hLYZ-TLN-</description><subject>BEER</subject><subject>BIOCHEMISTRY</subject><subject>CHEMISTRY</subject><subject>COMPOSITIONS THEREOF</subject><subject>CULTURE MEDIA</subject><subject>ENZYMOLOGY</subject><subject>HUMAN NECESSITIES</subject><subject>HYGIENE</subject><subject>MEDICAL OR VETERINARY SCIENCE</subject><subject>METALLURGY</subject><subject>MICROBIOLOGY</subject><subject>MICROORGANISMS OR ENZYMES</subject><subject>MUTATION OR GENETIC ENGINEERING</subject><subject>ORGANIC CHEMISTRY</subject><subject>PEPTIDES</subject><subject>PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES</subject><subject>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</subject><subject>SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS</subject><subject>SPIRITS</subject><subject>VINEGAR</subject><subject>WINE</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>2021</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNrjZAgL8fHTNbVQSMxLUcjwiYxSSE_NS1VIKy3OzM9TKCjKL0nNzANLJuaVZCYlJpekFmUm5igAGZllmSWVEKmCgpzM5MQSkJaSjNSi1Pw0HgbWtMSc4lReKM3NoOjmGuLsoZtakB-fWlyQmAy0piTe2c_Q0MjE1NzEwNLRmBg1AGIIOJw</recordid><startdate>20210309</startdate><enddate>20210309</enddate><creator>JIN TIANMING</creator><creator>CHEN TING</creator><creator>WANG XIAOYUE</creator><creator>ZHAO WEI</creator><creator>LAN ZHAOYU</creator><creator>LI DAN</creator><creator>FAN ZHENWEI</creator><creator>WU JINGQIAO</creator><creator>HE JINGWEN</creator><creator>YAO JINGLI</creator><scope>EVB</scope></search><sort><creationdate>20210309</creationdate><title>TLN-58 and hLYZ gene fusion protein and antibacterial activity and application thereof</title><author>JIN TIANMING ; CHEN TING ; WANG XIAOYUE ; ZHAO WEI ; LAN ZHAOYU ; LI DAN ; FAN ZHENWEI ; WU JINGQIAO ; HE JINGWEN ; YAO JINGLI</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_CN112457409A3</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>chi ; eng</language><creationdate>2021</creationdate><topic>BEER</topic><topic>BIOCHEMISTRY</topic><topic>CHEMISTRY</topic><topic>COMPOSITIONS THEREOF</topic><topic>CULTURE MEDIA</topic><topic>ENZYMOLOGY</topic><topic>HUMAN NECESSITIES</topic><topic>HYGIENE</topic><topic>MEDICAL OR VETERINARY SCIENCE</topic><topic>METALLURGY</topic><topic>MICROBIOLOGY</topic><topic>MICROORGANISMS OR ENZYMES</topic><topic>MUTATION OR GENETIC ENGINEERING</topic><topic>ORGANIC CHEMISTRY</topic><topic>PEPTIDES</topic><topic>PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES</topic><topic>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</topic><topic>SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS</topic><topic>SPIRITS</topic><topic>VINEGAR</topic><topic>WINE</topic><toplevel>online_resources</toplevel><creatorcontrib>JIN TIANMING</creatorcontrib><creatorcontrib>CHEN TING</creatorcontrib><creatorcontrib>WANG XIAOYUE</creatorcontrib><creatorcontrib>ZHAO WEI</creatorcontrib><creatorcontrib>LAN ZHAOYU</creatorcontrib><creatorcontrib>LI DAN</creatorcontrib><creatorcontrib>FAN ZHENWEI</creatorcontrib><creatorcontrib>WU JINGQIAO</creatorcontrib><creatorcontrib>HE JINGWEN</creatorcontrib><creatorcontrib>YAO JINGLI</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>JIN TIANMING</au><au>CHEN TING</au><au>WANG XIAOYUE</au><au>ZHAO WEI</au><au>LAN ZHAOYU</au><au>LI DAN</au><au>FAN ZHENWEI</au><au>WU JINGQIAO</au><au>HE JINGWEN</au><au>YAO JINGLI</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>TLN-58 and hLYZ gene fusion protein and antibacterial activity and application thereof</title><date>2021-03-09</date><risdate>2021</risdate><abstract>The invention relates to an hLYZ-TLN-58 gene fusion protein. The fusion protein is constructed by the following steps of (1) optimizing to obtain an hLYZ-TLN-58 gene SEQ ID NO.9 which is efficiently expressed in a mouse body according to a TLN-58 gene base sequence GI: AP02750 and an hLYZ gene standard sequence GI: NC_000012.10; (2) carrying out PCR amplification on the hLYZ-TLN-58 gene; and (3) expressing and purifying the recombinant prokaryotic expression vector pGEX-4T-1-hLYZ-TLN-58 to obtain the hLYZ-TLN-58 gene fusion protein. According to the fusion protein and antibacterial activity and the application thereof, the protein expressed by the recombinant prokaryotic plasmid constructed shows good bacteriostatic activity on an experimental strain, and a new treatment thought and experimental basis for clinical prevention and treatment of bacterial diseases is provided.
本发明涉及一种hLYZ-TLN-58基因融合蛋白，构建步骤如下：⑴根据TLN-58基因碱基序列GI：AP02750和hLYZ基因标准序列GI：NC_000012.10，优化得到在小鼠体内高效表达的hLYZ-TLN-58基因SEQ ID NO.9；⑵PCR扩增hLYZ-TLN-</abstract><oa>free_for_read</oa></addata></record> |
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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY HUMAN NECESSITIES HYGIENE MEDICAL OR VETERINARY SCIENCE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING ORGANIC CHEMISTRY PEPTIDES PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS SPIRITS VINEGAR WINE |
| title | TLN-58 and hLYZ gene fusion protein and antibacterial activity and application thereof |
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