SSR molecular marker related to cold resistance of bamboos, SSR primer and application of SSR molecular marker and SSR primer
The invention provides an SSR molecular marker related to cold resistance of bamboos, an SSR primer and an application of the SSR molecular marker and the SSR primer, and belongs to the technical field of molecular markers and detection thereof. Through transcriptome sequencing results, ICE1CBFCOR p...
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creator | LI SHANWEN REN FEI LI QINGHUA WU DEJUN YAO JUNXIU |
description | The invention provides an SSR molecular marker related to cold resistance of bamboos, an SSR primer and an application of the SSR molecular marker and the SSR primer, and belongs to the technical field of molecular markers and detection thereof. Through transcriptome sequencing results, ICE1CBFCOR pathway related genes are further screened, 38 unigenes related to cold-resistant markers are found in the related pathways, 38 pairs of SSR primers are synthesized according to SSR locus distribution characteristics in the unigenes, and through primer screening, 9 pairs of loci with good polymorphism are finally obtained and further verified. Finally, it is proved that the SSR molecular marker obtained through SSR primer amplification can be used for identifying the cold resistance of bamboo germplasm resources. The detection method can complete identification of the cold-resistant bamboos in a short time, has the advantages of high efficiency, accuracy, low cost, simplicity and conveniencein operation and the like |
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Through transcriptome sequencing results, ICE1CBFCOR pathway related genes are further screened, 38 unigenes related to cold-resistant markers are found in the related pathways, 38 pairs of SSR primers are synthesized according to SSR locus distribution characteristics in the unigenes, and through primer screening, 9 pairs of loci with good polymorphism are finally obtained and further verified. Finally, it is proved that the SSR molecular marker obtained through SSR primer amplification can be used for identifying the cold resistance of bamboo germplasm resources. 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Through transcriptome sequencing results, ICE1CBFCOR pathway related genes are further screened, 38 unigenes related to cold-resistant markers are found in the related pathways, 38 pairs of SSR primers are synthesized according to SSR locus distribution characteristics in the unigenes, and through primer screening, 9 pairs of loci with good polymorphism are finally obtained and further verified. Finally, it is proved that the SSR molecular marker obtained through SSR primer amplification can be used for identifying the cold resistance of bamboo germplasm resources. The detection method can complete identification of the cold-resistant bamboos in a short time, has the advantages of high efficiency, accuracy, low cost, simplicity and conveniencein operation and the like</abstract><oa>free_for_read</oa></addata></record> |
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language | chi ; eng |
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subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE |
title | SSR molecular marker related to cold resistance of bamboos, SSR primer and application of SSR molecular marker and SSR primer |
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