2-keto-L-gulonic acid producing strain and construction method thereof
The invention provides a construction method for a 2-keto-L-gulonic acid producing strain. The method comprises one or more of the following steps in any sequence: 1) interrupting a membrane-bound glucose dehydrogenase coding gene in a chromosome of tetramorium; 2) interrupting a glucokinase coding...
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| creator | YANG SHENG TAO RONGSHENG WANG QINGZHUO DIAO LIUYANG DUAN CHUNLAN LIU YINGMIAO JIANG YU |
| description | The invention provides a construction method for a 2-keto-L-gulonic acid producing strain. The method comprises one or more of the following steps in any sequence: 1) interrupting a membrane-bound glucose dehydrogenase coding gene in a chromosome of tetramorium; 2) interrupting a glucokinase coding gene; 3) interrupting a gluconokinase coding gene; 4) interrupting a 2-ketoaldose reductase coding gene and/or isozyme coding gene thereof; 5) carrying out recombinant expression on NAD(P)+ type glucose dehydrogenase; 6) carrying out recombinant expression on the NAD(P)H type 2,5-diketo-D-gluconatereductase; 7) carrying out overexpression on the 2,5-diketo-D-gluconic acid internal transport protein ARU92990 and ARU92994; and 8) interrupting the PTS component coding gene in the cell chromosome to obtain a genetically engineered bacterium. The 2-keto-L-gulonic acid producing strain constructed by the construction method can be used for producing 2-keto-L-gulonic acid by taking glucose as a raw material through a one- |
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The method comprises one or more of the following steps in any sequence: 1) interrupting a membrane-bound glucose dehydrogenase coding gene in a chromosome of tetramorium; 2) interrupting a glucokinase coding gene; 3) interrupting a gluconokinase coding gene; 4) interrupting a 2-ketoaldose reductase coding gene and/or isozyme coding gene thereof; 5) carrying out recombinant expression on NAD(P)+ type glucose dehydrogenase; 6) carrying out recombinant expression on the NAD(P)H type 2,5-diketo-D-gluconatereductase; 7) carrying out overexpression on the 2,5-diketo-D-gluconic acid internal transport protein ARU92990 and ARU92994; and 8) interrupting the PTS component coding gene in the cell chromosome to obtain a genetically engineered bacterium. 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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE |
| title | 2-keto-L-gulonic acid producing strain and construction method thereof |
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