Coding gene of soluble antrodia cinnamomea immunomodulatory protein, recombinant expression vector, engineering bacterium and application thereof
The invention relates to a coding gene of soluble antrodia camphorata immunomodulatory protein, a recombinant expression vector, engineering bacteria and application thereof. According to the invention, sequence optimization is carried out on a gene for encoding antrodia cinnamomea immunomodulatory...
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| creator | LI CAICAI YU MINGYU JI SONGSHAN ZHANG ZIQUN DONG BINGXUE YANG GUODONG ZHANG YINGJUN WU JIANFENG |
| description | The invention relates to a coding gene of soluble antrodia camphorata immunomodulatory protein, a recombinant expression vector, engineering bacteria and application thereof. According to the invention, sequence optimization is carried out on a gene for encoding antrodia cinnamomea immunomodulatory protein, an OACA gene is designed and synthesized, and a recombinant expression vector of the OACA gene is constructed; by introducing the vector into host bacteria, engineering bacteria capable of efficiently expressing soluble antrodia cinnamomea immunomodulatory protein are obtained. The fermentation conditions of engineering bacteria are further optimized, and a method for producing antrodia cinnamomea immunomodulatory protein by fermenting escherichia coli with corn steep liquor and alcoholfermentation waste liquor is provided. The engineering bacteria constructed by the invention not only can be used for soluble expression of antrodia camphorata immunomodulatory protein, but also havethe yield as high as 178. |
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According to the invention, sequence optimization is carried out on a gene for encoding antrodia cinnamomea immunomodulatory protein, an OACA gene is designed and synthesized, and a recombinant expression vector of the OACA gene is constructed; by introducing the vector into host bacteria, engineering bacteria capable of efficiently expressing soluble antrodia cinnamomea immunomodulatory protein are obtained. The fermentation conditions of engineering bacteria are further optimized, and a method for producing antrodia cinnamomea immunomodulatory protein by fermenting escherichia coli with corn steep liquor and alcoholfermentation waste liquor is provided. 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According to the invention, sequence optimization is carried out on a gene for encoding antrodia cinnamomea immunomodulatory protein, an OACA gene is designed and synthesized, and a recombinant expression vector of the OACA gene is constructed; by introducing the vector into host bacteria, engineering bacteria capable of efficiently expressing soluble antrodia cinnamomea immunomodulatory protein are obtained. The fermentation conditions of engineering bacteria are further optimized, and a method for producing antrodia cinnamomea immunomodulatory protein by fermenting escherichia coli with corn steep liquor and alcoholfermentation waste liquor is provided. The engineering bacteria constructed by the invention not only can be used for soluble expression of antrodia camphorata immunomodulatory protein, but also havethe yield as high as 178.</abstract><oa>free_for_read</oa></addata></record> |
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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING ORGANIC CHEMISTRY PEPTIDES PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE |
| title | Coding gene of soluble antrodia cinnamomea immunomodulatory protein, recombinant expression vector, engineering bacterium and application thereof |
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