Breeding method of densovirus-resistant silkworms
The invention discloses a breeding method of densovirus-resistant silkworms. The method comprises the following steps of an F1 generation, namely determining a Bmnsd-Z target point, constructing sgRNA-containing plasmid pXL [IE1-DsRed-U6-sgRNA], and leading the sgRNA-containing plasmid pXL [IE1-DsRe...
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| creator | LI MUWANG GAO YIHAN SUN XIA PANG HUILIN QIN SHENG WANG XUEYANG |
| description | The invention discloses a breeding method of densovirus-resistant silkworms. The method comprises the following steps of an F1 generation, namely determining a Bmnsd-Z target point, constructing sgRNA-containing plasmid pXL [IE1-DsRed-U6-sgRNA], and leading the sgRNA-containing plasmid pXL [IE1-DsRed-U6-sgRNA] into silkworm bodies; an F2 generation, namely selfing the F1 generation with the plasmid, and screening red fluorescent individuals to obtain F2 generation positive individuals; an F3 generation, namely hybridizing the F2 generation with silkworms expressing a Cas9 protein strain to obtain a bifluorescence offspring, and determining F3 generation positive individuals by performing primary screening on the disease resistance of the densovirus disease and genome identification; an F4generation, namely carrying out F3 generation selfing to screen fluorescence-free individuals, carrying out genome and disease resistance identification again, and determining a fluorescence-free target strain with densovirus |
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The method comprises the following steps of an F1 generation, namely determining a Bmnsd-Z target point, constructing sgRNA-containing plasmid pXL [IE1-DsRed-U6-sgRNA], and leading the sgRNA-containing plasmid pXL [IE1-DsRed-U6-sgRNA] into silkworm bodies; an F2 generation, namely selfing the F1 generation with the plasmid, and screening red fluorescent individuals to obtain F2 generation positive individuals; an F3 generation, namely hybridizing the F2 generation with silkworms expressing a Cas9 protein strain to obtain a bifluorescence offspring, and determining F3 generation positive individuals by performing primary screening on the disease resistance of the densovirus disease and genome identification; an F4generation, namely carrying out F3 generation selfing to screen fluorescence-free individuals, carrying out genome and disease resistance identification again, and determining a fluorescence-free target strain with densovirus</description><language>chi ; eng</language><subject>AGRICULTURE ; ANIMAL HUSBANDRY ; CARE OF BIRDS, FISHES, INSECTS ; FISHING ; FORESTRY ; HUMAN NECESSITIES ; HUNTING ; NEW BREEDS OF ANIMALS ; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR ; TRAPPING</subject><creationdate>2021</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20210219&DB=EPODOC&CC=CN&NR=112369376A$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,780,885,25564,76547</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20210219&DB=EPODOC&CC=CN&NR=112369376A$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>LI MUWANG</creatorcontrib><creatorcontrib>GAO YIHAN</creatorcontrib><creatorcontrib>SUN XIA</creatorcontrib><creatorcontrib>PANG HUILIN</creatorcontrib><creatorcontrib>QIN SHENG</creatorcontrib><creatorcontrib>WANG XUEYANG</creatorcontrib><title>Breeding method of densovirus-resistant silkworms</title><description>The invention discloses a breeding method of densovirus-resistant silkworms. 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| subjects | AGRICULTURE ANIMAL HUSBANDRY CARE OF BIRDS, FISHES, INSECTS FISHING FORESTRY HUMAN NECESSITIES HUNTING NEW BREEDS OF ANIMALS REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR TRAPPING |
| title | Breeding method of densovirus-resistant silkworms |
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