Sample treatment method, extraction kit and extraction device for rapidly extracting biological DNA
The invention provides a biological sample treatment method which is used for rapidly extracting DNA of biological tissues. The biological sample treatment method comprises the following steps: aftersimply treating a sample, grinding the sample by a grinding instrument; transferring the treated biol...
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| creator | ZHANG JUNFENG ZHANG HUAIYUAN DING WEIYUN |
| description | The invention provides a biological sample treatment method which is used for rapidly extracting DNA of biological tissues. The biological sample treatment method comprises the following steps: aftersimply treating a sample, grinding the sample by a grinding instrument; transferring the treated biological tissue sample into a DNA lysis solution; adding isopropanol to form nano DNA; transferring the nano DNA into a DNA micro-column; and carrying out washing and eluting. The biological sample treatment method provided by the invention is used for rapidly extracting the DNA of the biological tissues, so that the operation time can be greatly shortened, the operation steps are simplified and the experiment efficiency is improved; and a toxic reagent is not used so that the health of an operator is facilitated and damages to the environment are reduced.
本发明提供一种生物样品处理方法，用于生物组织DNA的快速提取，包括：样品的简单处理后，经过研磨仪的研磨，把处理后的生物组织样品转入DNA裂解液，加入异丙醇形成纳米DNA，转入DNA微柱，清洗和洗脱。本发明提供的一种生物样品处理方法，用于生物组织DNA的快速提取，可大大缩短操作时间，简化操作步骤，提高实验效率，且不使用有毒试剂 |
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本发明提供一种生物样品处理方法，用于生物组织DNA的快速提取，包括：样品的简单处理后，经过研磨仪的研磨，把处理后的生物组织样品转入DNA裂解液，加入异丙醇形成纳米DNA，转入DNA微柱，清洗和洗脱。本发明提供的一种生物样品处理方法，用于生物组织DNA的快速提取，可大大缩短操作时间，简化操作步骤，提高实验效率，且不使用有毒试剂</description><language>chi ; eng</language><subject>APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY ; BEER ; BIOCHEMISTRY ; CHEMISTRY ; COMPOSITIONS THEREOF ; CRUSHING, PULVERISING, OR DISINTEGRATING ; CRUSHING, PULVERISING, OR DISINTEGRATING IN GENERAL ; CULTURE MEDIA ; ENZYMOLOGY ; INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTEDFOR SPECIFIC APPLICATION FIELDS ; METALLURGY ; MICROBIOLOGY ; MICROORGANISMS OR ENZYMES ; MILLING GRAIN ; MUTATION OR GENETIC ENGINEERING ; PERFORMING OPERATIONS ; PHYSICS ; PREPARATORY TREATMENT OF GRAIN FOR MILLING ; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS ; SPIRITS ; TRANSPORTING ; VINEGAR ; WINE</subject><creationdate>2021</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20210122&DB=EPODOC&CC=CN&NR=112251431A$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,780,885,25563,76318</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20210122&DB=EPODOC&CC=CN&NR=112251431A$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>ZHANG JUNFENG</creatorcontrib><creatorcontrib>ZHANG HUAIYUAN</creatorcontrib><creatorcontrib>DING WEIYUN</creatorcontrib><title>Sample treatment method, extraction kit and extraction device for rapidly extracting biological DNA</title><description>The invention provides a biological sample treatment method which is used for rapidly extracting DNA of biological tissues. The biological sample treatment method comprises the following steps: aftersimply treating a sample, grinding the sample by a grinding instrument; transferring the treated biological tissue sample into a DNA lysis solution; adding isopropanol to form nano DNA; transferring the nano DNA into a DNA micro-column; and carrying out washing and eluting. The biological sample treatment method provided by the invention is used for rapidly extracting the DNA of the biological tissues, so that the operation time can be greatly shortened, the operation steps are simplified and the experiment efficiency is improved; and a toxic reagent is not used so that the health of an operator is facilitated and damages to the environment are reduced.
本发明提供一种生物样品处理方法，用于生物组织DNA的快速提取，包括：样品的简单处理后，经过研磨仪的研磨，把处理后的生物组织样品转入DNA裂解液，加入异丙醇形成纳米DNA，转入DNA微柱，清洗和洗脱。本发明提供的一种生物样品处理方法，用于生物组织DNA的快速提取，可大大缩短操作时间，简化操作步骤，提高实验效率，且不使用有毒试剂</description><subject>APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY</subject><subject>BEER</subject><subject>BIOCHEMISTRY</subject><subject>CHEMISTRY</subject><subject>COMPOSITIONS THEREOF</subject><subject>CRUSHING, PULVERISING, OR DISINTEGRATING</subject><subject>CRUSHING, PULVERISING, OR DISINTEGRATING IN GENERAL</subject><subject>CULTURE MEDIA</subject><subject>ENZYMOLOGY</subject><subject>INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTEDFOR SPECIFIC APPLICATION FIELDS</subject><subject>METALLURGY</subject><subject>MICROBIOLOGY</subject><subject>MICROORGANISMS OR ENZYMES</subject><subject>MILLING GRAIN</subject><subject>MUTATION OR GENETIC ENGINEERING</subject><subject>PERFORMING OPERATIONS</subject><subject>PHYSICS</subject><subject>PREPARATORY TREATMENT OF GRAIN FOR MILLING</subject><subject>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</subject><subject>SPIRITS</subject><subject>TRANSPORTING</subject><subject>VINEGAR</subject><subject>WINE</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>2021</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNqNijEOwjAMALswIOAPZochLTygKiCmLrBXJnFbiySOUgvB71kQYmQ66e7mhb1gSJ5AM6EGigqBdBS3AXpqRqssEe6sgNH9KkcPtgS9ZMiY2PnXt8YBbixeBrbo4dDWy2LWo59o9eGiWJ-O1-a8pSQdTQktRdKuaY0py73ZVaau_nnerPA-Xw</recordid><startdate>20210122</startdate><enddate>20210122</enddate><creator>ZHANG JUNFENG</creator><creator>ZHANG HUAIYUAN</creator><creator>DING WEIYUN</creator><scope>EVB</scope></search><sort><creationdate>20210122</creationdate><title>Sample treatment method, extraction kit and extraction device for rapidly extracting biological DNA</title><author>ZHANG JUNFENG ; ZHANG HUAIYUAN ; DING WEIYUN</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_CN112251431A3</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>chi ; eng</language><creationdate>2021</creationdate><topic>APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY</topic><topic>BEER</topic><topic>BIOCHEMISTRY</topic><topic>CHEMISTRY</topic><topic>COMPOSITIONS THEREOF</topic><topic>CRUSHING, PULVERISING, OR DISINTEGRATING</topic><topic>CRUSHING, PULVERISING, OR DISINTEGRATING IN GENERAL</topic><topic>CULTURE MEDIA</topic><topic>ENZYMOLOGY</topic><topic>INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTEDFOR SPECIFIC APPLICATION FIELDS</topic><topic>METALLURGY</topic><topic>MICROBIOLOGY</topic><topic>MICROORGANISMS OR ENZYMES</topic><topic>MILLING GRAIN</topic><topic>MUTATION OR GENETIC ENGINEERING</topic><topic>PERFORMING OPERATIONS</topic><topic>PHYSICS</topic><topic>PREPARATORY TREATMENT OF GRAIN FOR MILLING</topic><topic>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</topic><topic>SPIRITS</topic><topic>TRANSPORTING</topic><topic>VINEGAR</topic><topic>WINE</topic><toplevel>online_resources</toplevel><creatorcontrib>ZHANG JUNFENG</creatorcontrib><creatorcontrib>ZHANG HUAIYUAN</creatorcontrib><creatorcontrib>DING WEIYUN</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>ZHANG JUNFENG</au><au>ZHANG HUAIYUAN</au><au>DING WEIYUN</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>Sample treatment method, extraction kit and extraction device for rapidly extracting biological DNA</title><date>2021-01-22</date><risdate>2021</risdate><abstract>The invention provides a biological sample treatment method which is used for rapidly extracting DNA of biological tissues. The biological sample treatment method comprises the following steps: aftersimply treating a sample, grinding the sample by a grinding instrument; transferring the treated biological tissue sample into a DNA lysis solution; adding isopropanol to form nano DNA; transferring the nano DNA into a DNA micro-column; and carrying out washing and eluting. The biological sample treatment method provided by the invention is used for rapidly extracting the DNA of the biological tissues, so that the operation time can be greatly shortened, the operation steps are simplified and the experiment efficiency is improved; and a toxic reagent is not used so that the health of an operator is facilitated and damages to the environment are reduced.
本发明提供一种生物样品处理方法，用于生物组织DNA的快速提取，包括：样品的简单处理后，经过研磨仪的研磨，把处理后的生物组织样品转入DNA裂解液，加入异丙醇形成纳米DNA，转入DNA微柱，清洗和洗脱。本发明提供的一种生物样品处理方法，用于生物组织DNA的快速提取，可大大缩短操作时间，简化操作步骤，提高实验效率，且不使用有毒试剂</abstract><oa>free_for_read</oa></addata></record> |
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| subjects | APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CRUSHING, PULVERISING, OR DISINTEGRATING CRUSHING, PULVERISING, OR DISINTEGRATING IN GENERAL CULTURE MEDIA ENZYMOLOGY INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTEDFOR SPECIFIC APPLICATION FIELDS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MILLING GRAIN MUTATION OR GENETIC ENGINEERING PERFORMING OPERATIONS PHYSICS PREPARATORY TREATMENT OF GRAIN FOR MILLING PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS TRANSPORTING VINEGAR WINE |
| title | Sample treatment method, extraction kit and extraction device for rapidly extracting biological DNA |
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